Sequencing the genome of the powdery mildew fungus Blumeria graminis

对白粉病真菌 Blumeria graminis 的基因组进行测序

• 批准号: BB/E002803/1
• 负责人: Nicholas Talbot
• 金额: $ 19.98万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE002803%2F1
• 关键词: Sequencing; genome; powdery; mildew; fungus

This proposal aims at obtaining an annotated genome sequence of the powdery mildew fungus Blumeria graminis f sp hordei. This fungus is an obligate biotrophic pathogen that causes one the agronomically most important diseases of barley and is a 'model' organism for the study of powdery mildew diseases in other crops. We will sequence the genome with a 7-fold coverage. The sequence will be assembled automatically. An EST library of cDNAs isolated from haustoria will also be sequenced and annotated; this will complement the existing collections and complete the coverage of all the important stages in the pathogen's life cycle. The ESTs are instrumental in training the gene finding processes that drive the annotation. The annotation of the genomic sequence will be carried out using a series of pipelines already used for other genome projects (Botrytis and tomato). The results from the semi-automated annotation will then be verified by manual curation and reviewed by specialist researchers in the sequencing consortium. The results will be placed on publicly accessible web-sites and a meeting will be convened with the world wide user community to bring train potential users, present major outcomes of the sequencing and the first annotations and discuss the results.

该提案旨在获得白粉病真菌 Blumeria graminis f sp hordei 的注释基因组序列。这种真菌是一种专性生物营养病原体，可引起大麦农艺学上最重要的病害之一，并且是研究其他作物白粉病的“模型”生物。我们将以 7 倍覆盖率对基因组进行测序。该序列将自动组装。从吸器中分离的 EST cDNA 文库也将被测序和注释；这将补充现有的馆藏，并完成对病原体生命周期所有重要阶段的覆盖。 EST 有助于训练驱动注释的基因查找过程。基因组序列的注释将使用一系列已用于其他基因组项目（葡萄孢和番茄）的管道进行。然后，半自动注释的结果将通过手动管理进行验证，并由测序联盟的专业研究人员进行审查。结果将发布在可公开访问的网站上，并将与全球用户社区召开会议，培训潜在用户，展示测序的主要结果和第一个注释并讨论结果。

项目成果

Comparative genome analysis reveals an absence of leucine-rich repeat pattern-recognition receptor proteins in the kingdom Fungi.

• DOI: 10.1371/journal.pone.0012725
• 发表时间: 2010-09-14
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Soanes DM;Talbot NJ
• 通讯作者: Talbot NJ