Comparative cDNA Sequencing in Radish (Raphanus), a Crop, Weed, and Model System in Ecology and Evolution

萝卜属（Raphanus）植物有两种：萝卜R. sativus为食用萝卜，R.萝卜属是野生品种。野生物种很重要，因为它可能是作物的祖先，它是世界范围内日益严重的作物杂草，并且因为野生和作物萝卜之间的杂交种已经成为加州自然土地的入侵物种。 萝卜也是生态学和进化的模式系统，特别是植物-动物相互作用和自然选择的研究。 尽管它作为作物、杂草和研究物种的重要性，但几乎没有萝卜的基因序列数据，这严重限制了对萝卜生物学的理解。 该项目将从作物和野生萝卜物种中产生数千个基因序列，并产生数百个遗传标记。 这些序列和标记将使下一代的发现，包括遗传基础：作物驯化，杂草和入侵物种的进化，适应传粉者，食草动物和全球变化。 将产生的基因序列与来自同一家族中其他物种（作物属芸苔属和模式植物拟南芥）的现有序列进行比较，将促进我们对基因序列如何进化的理解。 获得项目成果所产生的序列、标记和克隆将通过拟南芥生物资源中心（GenBank）免费提供给更广泛的研究团体。（ABRC：http：//www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm），植物基因组数据库（PlantGDB：http：//www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm），拟南芥信息资源（TAIR：http：//www.arabidopsis.org/）和基因组研究所网站（TIGR：http：//www.tigr.org/）和密歇根州立大学（MSU：http：//www.kbs.msu.edu/Faculty/Conner/Index.htm）。 MSU网站将以协作内容创建（wiki）为特色，以促进全球萝卜研究社区之间的思想和数据交流。

There are two species in the plant genus Raphanus: R. sativus is the edible radish and R. raphanistrum is the wild species. The wild species is important because it is the likely ancestor of the crop, it is an increasingly serious crop weed worldwide, and because hybrids between the wild and crop radish have become an invasive species of natural land in California. Radish is also a model system in ecology and evolution, especially for studies of plant-animal interactions and natural selection. Despite its importance as a crop, weed, and study species, almost no gene sequence data is available for radish, severely limiting future progress in understanding the biology of radish. This project will produce sequences of thousands of genes from both the crop and wild radish species and generate hundreds of genetic markers. These sequences and markers will enable the next generation of discoveries, including the genetic basis of: crop domestication, weed and invasive species evolution, adaptation to pollinators, herbivores, and global change. Comparisons of the gene sequences generated to existing sequences from other species in the same family (the crop genus Brassica and the model plant Arabidopsis) will advance our understanding of how gene sequences evolve. Access to project outcomesThe sequences, markers, and clones produced will be made freely available to the broader research community via GenBank, the Arabidopsis Biological Resource Center (ABRC: http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm), Plant Genome Database (PlantGDB: http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/abrchome.htm), the Arabidopsis Information Resource (TAIR: http://www.arabidopsis.org/) and websites at the Institute for Genomic Research (TIGR: http://www.tigr.org/) and Michigan State University (MSU: http://www.kbs.msu.edu/Faculty/Conner/Index.htm). The MSU website will feature collaborative content creation (wiki) to facilitate the exchange of ideas and data among the worldwide radish research community.