Arabidopsis as a New Experimental Platform to Investigate the Function of the Nuclear Pore Protein Tpr in SUMOylation and mRNA export.
拟南芥作为新的实验平台来研究核孔蛋白 Tpr 在 SUMO 化和 mRNA 输出中的功能。
基本信息
- 批准号:0641271
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing grant
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-04-01 至 2011-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Intellectual merit. The nuclear pore complex (NPC) is the gatekeeper for traffic between the nucleus and the cytoplasm of the cell. The NPC structure and protein content is conserved among eukaryotes. Studies of the NPC in several eukaryotic organisms suggest that the long coiled-coil Tpr protein has an important role in mRNA export. Dr. Meier has identified an allelic series of three mutants of the Arabidopsis Tpr gene which show a concomitant increase in severity of three phenotypes i.e. early flowering, Increased SUMOylated protein accumulation, and nuclear accumulation of poly(A)+ RNA. SUMOylation is a post-translational modification of proteins with the polypeptide SUMO. Post-translation modification is a regulatory mechanism that alters the properties of proteins. Dr. Meier has shown that AtTpr and an Arabidopsis SUMO protease interact physically and genetically. Three specific aims will investigate the function of AtTpr at the nuclear pore. They are:1. To show that AtTpr anchors the SUMO protease to the nuclear pore and influences its biological activity;2. To use a proteomics approach to purify AtTpr complexes to determine whether components of the mRNA export machinery are present; 3. To use a genetic screen for suppressors and enhancers of a tpr mutant allele to identify new components connecting flowering time, SUMO homeostasis and mRNA export. This project utilizes the first available multicellular system to study the effects of Tpr mutants on development, likely through the anchoring of nuclear pore-associated activities. Broader Impact. As part of this project, database management, education, and outreach activities will be performed in Dr. Meyer's laboratory. A searchable database for Arabidopsis long coiled-coil proteins ARABI-COIL is widely available to the scientific community listed at TAIR and will be continuously curated. During the past three years, a new bioinformatics module for a plant molecular biology course has been developed and the PI has participated in a local Functional Genomics Summer Course targeting students and faculty from Ohio colleges. These or similar activities will continue in the proposed funding period. The PI is continuously offering an online course for the general public on Plant Biotechnology. Undergraduate students, graduate students and postdocs will be trained and will present their data at national and international meetings, in peer-reviewed publications and in review articles.
知识价值。核孔复合体(NPC)是细胞核和细胞质之间交通的守门人。NPC的结构和蛋白质含量在真核生物中是保守的。对几种真核生物鼻咽癌的研究表明,长卷曲的Tpr蛋白在mRNA输出中起重要作用。Meier博士已经确定了拟南芥Tpr基因的三个突变的等位基因系列,这些突变显示出伴随三种表型的严重程度增加,即早期开花,增加SUMOylated蛋白积累和核聚(a)+ RNA积累。SUMO基化是蛋白质翻译后的修饰,含有多肽SUMO。翻译后修饰是一种改变蛋白质特性的调控机制。Meier博士已经证明,AtTpr和拟南芥SUMO蛋白酶在生理和基因上相互作用。三个具体目标将研究AtTpr在核孔中的作用。它们是:1。证明AtTpr将SUMO蛋白酶锚定在核孔上并影响其生物活性;使用蛋白质组学方法纯化AtTpr复合物,以确定mRNA输出机制的成分是否存在;3. 利用基因筛选tpr突变等位基因的抑制子和增强子,鉴定与开花时间、SUMO稳态和mRNA输出相关的新成分。该项目利用第一个可用的多细胞系统来研究Tpr突变体对发育的影响,可能是通过锚定核孔相关活动。更广泛的影响。作为该项目的一部分,数据库管理、教育和外展活动将在Meyer博士的实验室进行。一个可搜索的拟南芥长卷曲蛋白数据库ARABI-COIL已广泛提供给TAIR列出的科学界,并将不断进行整理。在过去的三年里,一个新的植物分子生物学课程的生物信息学模块已经开发出来,PI还参加了一个针对俄亥俄州大学学生和教师的当地功能基因组学暑期课程。这些或类似的活动将在拟议的筹资期内继续进行。PI不断为公众提供关于植物生物技术的在线课程。本科生、研究生和博士后将接受培训,并将在国内和国际会议、同行评审出版物和评论文章中展示他们的数据。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Iris Meier其他文献
DOI 10.1007/s00425-004-1215-8 ORIGINAL ARTICLE
DOI 10.1007/s00425-004-1215-8 原始文章
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
Sun Yong;Jeong Ae;Nancy Peffer Ae;Iris Meier - 通讯作者:
Iris Meier
Elicitor-inducible and constitutive in vivo DNA footprints indicate novel cis-acting elements in the promoter of a parsley gene encoding pathogenesis-related protein 1.
诱导子诱导型和组成型体内 DNA 足迹表明编码发病机制相关蛋白 1 的欧芹基因启动子中存在新的顺式作用元件。
- DOI:
10.1105/tpc.3.3.309 - 发表时间:
1991 - 期刊:
- 影响因子:0
- 作者:
Iris Meier;Klaus Hahlbrock;I. Somssich - 通讯作者:
I. Somssich
Disruption of the gene encoding the ubiquitin-conjugating enzyme UbcM4 has no effect on proliferation and in vitro differentiation of mouse embryonic stem cells.
破坏编码泛素结合酶 UbcM4 的基因对小鼠胚胎干细胞的增殖和体外分化没有影响。
- DOI:
10.1016/s0167-4781(00)00221-9 - 发表时间:
2000 - 期刊:
- 影响因子:0
- 作者:
Ekaterini Pringa;Iris Meier;U. Müller;G. Martinez;Klaus Harbers - 通讯作者:
Klaus Harbers
Iris Meier的其他文献
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{{ truncateString('Iris Meier', 18)}}的其他基金
A Molecular Role for Plant LINC complexes in Pollen Tube and Guard Cell Function.
植物 LINC 复合物在花粉管和保卫细胞功能中的分子作用。
- 批准号:
2023348 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Standard Grant
Function and mechanism of action of plant-specific LINC complexes in pollen tube and guard cell biology.
植物特异性 LINC 复合物在花粉管和保卫细胞生物学中的功能和作用机制。
- 批准号:
1613501 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Standard Grant
Towards a Plant Nuclear Envelope Interactome
迈向植物核膜相互作用组
- 批准号:
1243844 - 财政年份:2013
- 资助金额:
-- - 项目类别:
Continuing Grant
Adaptation of RanGAP localization and function for plant-specific development
RanGAP 本地化和功能的适应,用于植物特定的开发
- 批准号:
0919880 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Continuing Grant
Group Travel Proposal: Nuclear Envelope and Golgi, Marseille 2008
团体旅行提案:核膜和高尔基体,马赛 2008 年
- 批准号:
0805009 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Standard Grant
Investigating Structure, Function, and Evolution of a Plant-Specific Nuclear Envelope Targeting Domain.
研究植物特异性核膜靶向结构域的结构、功能和进化。
- 批准号:
0343167 - 财政年份:2004
- 资助金额:
-- - 项目类别:
Continuing Grant
Arabidopsis 2010: Investigating Coiled-coil Proteins in the Arabidopsis ORFeome
拟南芥 2010:研究拟南芥 ORFeome 中的卷曲螺旋蛋白
- 批准号:
0209339 - 财政年份:2002
- 资助金额:
-- - 项目类别:
Standard Grant
Investigating the Role of Novel Nuclear Envelope-Associated Plant Proteins in Nuclear Dynamics
研究新型核膜相关植物蛋白在核动力学中的作用
- 批准号:
0079577 - 财政年份:2001
- 资助金额:
-- - 项目类别:
Continuing grant
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