Towards a Plant Nuclear Envelope Interactome

迈向植物核膜相互作用组

• 批准号: 1243844
• 负责人: Iris Meier
• 金额: $ 84.68万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1243844&HistoricalAwards=false
• 关键词: Towards; Plant; Nuclear; Envelope; Interactome

The proteins of the plant nuclear envelope have important roles in many cell functions, including mechanical signaling, controlling the shape and movement of the nucleus, and the cell cycle. However, for reasons that are not currently understood, the protein composition of the plant nuclear envelope differs significantly from that of the animals and fungi. The goal of this project is to understand the function of the novel plant-specific proteins that span the nuclear envelope, and which are predicted to play roles in plant development, pollination, and the responses to light and pathogens. The experimental approaches will include live-cell imaging by light microscopy, and the identification of the protein interaction partners using techniques of affinity chromatography, tandem mass spectrometry and computational biology. Broader Impacts. A novel course in biology and art will be developed, in collaboration with an Art professor at Ohio State University, with the goal to make topics in contemporary plant biology accessible and comprehensible to the public. A public Bio-Art exhibit will be developed and the course will form the basis for a module that can be used by high school teachers.

植物核膜蛋白在许多细胞功能中具有重要作用，包括机械信号，控制核的形状和运动以及细胞周期。然而，由于目前尚不清楚的原因，植物核膜的蛋白质组成与动物和真菌的蛋白质组成显著不同。该项目的目标是了解跨越核膜的新型植物特异性蛋白的功能，预计这些蛋白在植物发育，授粉以及对光和病原体的反应中发挥作用。实验方法将包括通过光学显微镜进行活细胞成像，以及使用亲和色谱、串联质谱和计算生物学技术鉴定蛋白质相互作用伙伴。更广泛的影响。将与俄亥俄州州立大学的一位艺术教授合作，开发一门生物学和艺术方面的新课程，目标是使公众能够接触和理解当代植物生物学的主题。将开发一个公共生物艺术展览，该课程将成为高中教师可以使用的模块的基础。