CAREER: Membrane Platform Technologies for Channel Protein Science and Sensing

职业：通道蛋白科学和传感的膜平台技术

• 批准号: 0644442
• 负责人: Jacob Schmidt
• 金额: $ 40万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-15 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0644442&HistoricalAwards=false
• 关键词: CAREER; Membrane; Platform; Technologies; Channel

Jacob Schmidt0644442The current technologies for creating freestanding planar lipid bilayer membranes in the laboratory are essentially unchanged over the past 30 years. Scientific studies, clinical research, and technological applications of channel proteins would all benefit greatly from a robust, long-lived, compact, and easily operated membrane platform. The objective of this research is to address these technological needs through the development of two innovations: (1) long-lived, mechanically stabilized membranes and (2) microfluidic systems capable of forming membranes quickly and automatically in an on-demand manner. Preliminary work by the principal investigator's (PI's) laboratory has shown significant improvements in mechanical stability and longevity of a free-standing lipid bilayer membrane can be achieved by encapsulating the membrane within an in situ photo-polymerized hydrogel. In the proposed work, the gel properties will be varied to optimize durability and extend the lifetime of the encapsulated membranes. The PI's laboratory has also developed a novel method of automated membrane formation in a microfluidic device driven by the solvent extraction properties of polydimethylsiloxane. In the proposed work, the existing prototype device will be optimized and generation device--consisting of a planar array of membranes individually addressable through multiplexed channels--will be developed and constructed. In addition to training graduate students, the PI is part of a number of programs to attract and host high school and undergraduate students in his laboratory.

雅各布·施密特·0644442目前在实验室中制造独立平面类脂双层膜的技术在过去30年中基本上没有变化。通道蛋白的科学研究、临床研究和技术应用都将从一个坚固、持久、紧凑和易于操作的膜平台中受益匪浅。这项研究的目的是通过开发两项创新来满足这些技术需求：(1)长寿命、机械稳定的膜和(2)能够以按需方式快速、自动地形成膜的微流控系统。首席研究员实验室的初步工作表明，通过将膜包裹在原位光聚合水凝胶中，可以显著提高自立式脂质双层膜的机械稳定性和寿命。在拟议的工作中，凝胶特性将发生变化，以优化胶囊膜的耐用性并延长其使用寿命。PI的实验室还开发了一种在微流控设备中自动成膜的新方法，该方法由聚二甲基硅氧烷的溶剂萃取特性驱动。在拟议的工作中，现有的原型装置将得到优化，发电装置--由可通过多路复用通道单独寻址的平面膜阵列--将被开发和建造。除了培训研究生，PI还是一系列项目的一部分，以吸引和接待他实验室的高中生和本科生。