IDBR: Simultaneous Tracking of Multiple Particles in Confocal Microscopy

IDBR：在共焦显微镜中同时跟踪多个粒子

• 批准号: 0649823
• 负责人: Sean Andersson
• 金额: $ 27.32万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649823&HistoricalAwards=false
• 关键词: IDBR; Simultaneous; Tracking; Multiple; Particles

This award is for the development of techniques for tracking multiple fluorescing particles simultaneously in a confocal microscope. The project will initially develop algorithms for tracking single fluorescent particles in vitro and inside living cells. Techniques from modern systems and control theory, including limited communication control and optimal estimation, will be built upon to enable tracking of multiple particles simultaneously. The capabilities to be introduced by this project will enable the study of communication between cells, processes inside living cells, and the dynamics of individual molecules and of molecular interactions. The ability to obtain three-dimensional trajectory information at fine temporal resolution is a vital component for developing a deeper understanding of many processes in molecular biology. The project is multi-disciplinary with both theoretical and experimental components. In addition to support for a female graduate student, modules for a pre-university outreach program will be developed and utilized in a university program targeting academically at-risk students in the 7th and 8th grades. Taking advantage of the large number of biotechnology companies in the greater Boston area, a new freshman level course will be developed for exploring emerging areas of science and technology.

该奖项是为了表彰在共聚焦显微镜中同时跟踪多个荧光颗粒的技术开发。该项目最初将开发用于在体外和活细胞内跟踪单个荧光颗粒的算法。来自现代系统和控制理论的技术，包括有限通信控制和最优估计，将建立在同时跟踪多个粒子的基础上。该项目引入的能力将使研究细胞之间的通信，活细胞内的过程，以及单个分子和分子相互作用的动力学成为可能。以精细的时间分辨率获得三维轨迹信息的能力是深入了解分子生物学中许多过程的重要组成部分。该项目是多学科的理论和实验组成部分。除了支助一名女研究生外，还将开发大学预科外联方案模块，并将其用于一项针对七年级和八年级学业有问题学生的大学方案。利用大波士顿地区大量的生物技术公司，将开发一个新的大一课程，以探索新兴的科学和技术领域。