IDBR: Simultaneous Tracking of Multiple Particles in Confocal Microscopy

该奖项是对在共聚焦显微镜中同时跟踪多个荧光粒子的技术的开发。该项目最初将开发在体外和活细胞内追踪单个荧光颗粒的算法。现代系统和控制理论的技术，包括有限通信控制和最优估计，将被建立在能够同时跟踪多个粒子的基础上。该项目将引入的能力将使研究细胞之间的通信、活细胞内的过程以及单个分子和分子相互作用的动力学成为可能。获得精细时间分辨率的三维轨迹信息的能力是加深对分子生物学中许多过程的理解的重要组成部分。该项目是多学科的，既有理论部分，也有实验部分。除了对女研究生的支持外，还将开发大学前外展计划的模块，并将其用于一项针对7年级和8年级学业风险学生的大学计划。利用大波士顿地区大量生物技术公司的优势，将开发一门新的大一水平课程，以探索新兴的科学和技术领域。

This award is for the development of techniques for tracking multiple fluorescing particles simultaneously in a confocal microscope. The project will initially develop algorithms for tracking single fluorescent particles in vitro and inside living cells. Techniques from modern systems and control theory, including limited communication control and optimal estimation, will be built upon to enable tracking of multiple particles simultaneously. The capabilities to be introduced by this project will enable the study of communication between cells, processes inside living cells, and the dynamics of individual molecules and of molecular interactions. The ability to obtain three-dimensional trajectory information at fine temporal resolution is a vital component for developing a deeper understanding of many processes in molecular biology. The project is multi-disciplinary with both theoretical and experimental components. In addition to support for a female graduate student, modules for a pre-university outreach program will be developed and utilized in a university program targeting academically at-risk students in the 7th and 8th grades. Taking advantage of the large number of biotechnology companies in the greater Boston area, a new freshman level course will be developed for exploring emerging areas of science and technology.