IDBR: An Automated, High-Throughput Micro System for Precision Imaging and Ablation of Cells

本文提出了一种用于高通量活细胞自动成像和激光消融的微流控装置。激光烧蚀是一种从样品表面去除材料的精确方法。通过自动激光消融细胞，该设备将减少系统用户偏差。该装置直接用于线虫的研究，也适用于斑马鱼胚胎和果蝇等系统。整个生物体的活体显微镜和细胞的物理消融是生物学中两种重要的技术，特别是在发育和神经科学领域。该奖项的设备设计将向公众开放，可以很容易地适应不同的应用。研究生、本科生和高中生将参与测试该设备，以提高其用户友好性，并拓宽高中生的体验。

This is a proposal to develop a microfluidic device for automated high-throughput live-cell imaging and laser ablation. Laser ablation is a precise method for removing material from the surface of the sample. By automating laser ablation of cells, the device will decrease systematic user biases. The device is directed toward research on the worm C. elegans and is applicable also to systems such as zebrafish embryos and Drosophila.Live microscopy of whole organisms and physical ablation of cells are two important techniques in biology, especially in the areas of development and neuroscience. The device designs from this award will be made available to the public and can be easily adapted for different applications. Graduate students, undergraduate students, and high school students will be involved in testing the device, to improve its user-friendliness and to broaden the experience of the high school students involved.