IDBR: An Automated, High-Throughput Micro System for Precision Imaging and Ablation of Cells

IDBR：用于细胞精确成像和消融的自动化、高通量微型系统

• 批准号: 0649833
• 负责人: Hang Lu
• 金额: --
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649833&HistoricalAwards=false
• 关键词: IDBR; Automated; Throughput; Micro; System

This is a proposal to develop a microfluidic device for automated high-throughput live-cell imaging and laser ablation. Laser ablation is a precise method for removing material from the surface of the sample. By automating laser ablation of cells, the device will decrease systematic user biases. The device is directed toward research on the worm C. elegans and is applicable also to systems such as zebrafish embryos and Drosophila.Live microscopy of whole organisms and physical ablation of cells are two important techniques in biology, especially in the areas of development and neuroscience. The device designs from this award will be made available to the public and can be easily adapted for different applications. Graduate students, undergraduate students, and high school students will be involved in testing the device, to improve its user-friendliness and to broaden the experience of the high school students involved.

这是一个建议，开发一个自动化的高通量活细胞成像和激光烧蚀的微流体装置。 激光烧蚀是从样品表面去除材料的精确方法。 通过自动激光消融细胞，该设备将减少系统性用户偏见。 该设备是针对蠕虫C的研究。整个生物体的活体显微镜和细胞的物理消融是生物学中的两种重要技术，特别是在发育和神经科学领域。该奖项的设备设计将向公众提供，并可以轻松地适应不同的应用。研究生、本科生和高中生将参与测试该设备，以提高其用户友好性，并拓宽高中生的经验。