Basophils as modulators of humoral and cellular allo-immune responses

我们已经证明，在记忆免疫反应中，嗜碱性细胞能够在其表面结合大量完整的抗原。这种结合是由抗原特异性IgE分子和嗜碱性细胞上的高亲和力IgE受体介导的。在再次暴露于抗原后，嗜碱性细胞被激活并构成脾脏和骨髓中IL-4和IL-6的主要来源。免疫和感染模型显示，活化的嗜碱性粒细胞显著改变CD4+ T细胞的表型，并在记忆免疫反应的发展中发挥重要作用。记忆型免疫反应在移植中占主导地位，特别是在急性排斥或慢性移植排斥后。我们假设，在急性和慢性移植排斥反应中，嗜碱性粒细胞作为体液和细胞免疫反应的调节剂发挥重要作用，并且嗜碱性粒细胞的激活是持续记忆免疫反应的良好指标。这些方面将在体外使用MLR反应、小鼠移植模型和移植后患者中进行研究。

We have shown that during memory immune responses basophils are able to bind significant amounts of intact antigens on their surface. This binding is mediated by antigen-specific IgE molecules and high affinity IgE receptors on basophils. After re-exposure to antigens basophils become activated and constitute the main source of IL-4 and IL-6 in the spleen and bone marrow. Activated basophils markedly alter the phenotype of CD4+ T cells and play an important role in the development of memory immune responses as shown in models of immunization and infection. A memory type immune response predominates in transplantation, especially after acute rejections or in the setting of chronic transplant rejection. We hypothesize that basophils play an important role as modulators of humoral and cellular immune responses during acute and chronic transplant rejection and that activation of basophils is a good indicator for ongoing memory immune responses. These aspects will be investigated in vitro using MLR reactions, in mouse models of transplantation, and in patients after transplantation.