Vascular Pattern Formation in Leaves of Arabidopsis and Rice

主要研究者：Timothy纳尔逊，Yale UniversityProposal IOS-0718881 Vascular Pattern Formation in Leaves of Aromopsis这个项目旨在了解植物叶片脉序网络的模式。生长素信号传导和生长素极性运输（PAT）的作用最近已被证明在许多发育过程中，包括静脉图案和叶序，这是茎上的叶的排列。然而，生长素模式的每种情况都依赖于不同的背景;某些细胞和其中的某些途径以该过程特有的方式，在发育的正确时间和地点产生生长素反应。也就是说，生长素作用的上游和下游都有独特的特征。该项目的先前结果表明，肌醇信号调节静脉图案。该项目将使用遗传学，细胞生物学和生物化学来测试以下假设：特定的磷酸肌醇信号提供了发育中的叶组织中的细胞空间信息与促进该组织中的静脉模式的更具体的PAT现象之间的联系。结果将包括与PAT相关的内膜交通机械的肌醇酶的共定位，磷酸肌醇信号和内膜交通蛋白之间的调节相互作用的定义，并在血管细胞中的活性肌醇底物和途径成分的鉴定。随着我们对静脉图案化和分化的理解的增加，它将提供一种影响发育、发病机制防御和生理学的手段（例如，C4光合作用、资源分配、养分吸收等），所有这些都依赖于脉。更广泛的影响：该项目作为学生，博士后科学家和少数民族暑期研究实习生的培训工具。该项目是一个来源的插图为PI的生物教学的非科学本科生，其中许多人有兴趣在科学政策和科学社会问题的工作。

PI: Timothy Nelson, Yale UniversityProposal IOS-0718881Vascular Pattern Formation in Leaves of ArabidopsisThis project seeks to understand the patterning of leaf venation networks in plant. A role for auxin signaling and polar auxin transport (PAT) has been demonstrated recently in many developmental processes, including vein patterning and phyllotaxy, which is the arrangement of leaves on a stem. However, each case of auxin patterning relies on a distinct context; certain cells and certain pathways within them are made auxin-responsive in a manner unique to that process, at the right time and place in development. That is, there are unique features both upstream and downstream of auxin''s role. Prior results from this project suggest that inositol signaling regulates vein patterning. This project will use genetics, cell biology and biochemistry to test the hypothesis that specific phosphoinositol signals provide a connection between cellular spatial information in developing leaf tissue and the more specific PAT phenomena that facilitate vein patterns in that tissue. The results would include the colocalization of inositol enzymes with the endomembrane traffic machinery associated with PAT, the definition of regulatory interactions among phosphoinositol signals and endomembrane traffic proteins, and the identification of active inositol substrates and pathway components in vascular cells. As our understanding of vein patterning and differentiation increases, it will provide a means to influence development, pathogenesis defense, and physiology (e.g., C4 photosynthesis, resource allocation, nutrient uptake, etc), all of which depend on veins. Broader Impacts: This project serves as a training vehicle for students, postdoctoral scientists, and minority summer research interns. The project is a source of illustrations for the PI''s teaching of biology to non-science undergraduates, many of whom have interest in working on science policy and science & society issues.