Analysis of Vascular Pattern Formation in Leaves of Arabidopsis

拟南芥叶片维管束模式形成分析

• 批准号: 0110730
• 负责人: Timothy Nelson
• 金额: $ 39万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0110730&HistoricalAwards=false
• 关键词: Analysis; Vascular; Pattern; Formation; Leaves

0110370NelsonThe components of leaf vascular pattern formation will be analyzed, using a molecular genetic approach in Arabidopsis. The formation of the leaf vascular pattern has developmental consequences beyond the simple siting of veins. Provascular sites in the leaf primordium, where the precursors of vein cells are formed, appear to serve as morphogenetic centers that regulate the location and specialization of vascular and other leaf cell types. This makes functional sense, since many of the cell types must function in cooperation with the veins, requiring a defined spatial relationship. Using forward and reverse genetic approaches, potential components of this process have been identified, including likely members of provascular signaling pathways. Their roles will be analyzed and additional candidates will be characterized. These are the aims:1) To identify the immediate signaling pathway partners of the VH1 provascular receptor-like kinase, which is a candidate for a key role in organizing vascular regions. 2) To determine the role of the cvp1 sterol methyl transferase (SMT2) in provascular cell siting or differentiation. These processes are defective in cvp1 mutants. 3) To determine the role of the SAUR gene represented by the thick vein mutant. This may represent a direct link between auxin signals and vein formation.4) To characterize the vein patterning roles of additional genes identified by mutants with vein pattern defects.

0110370尼尔森将使用拟南芥的分子遗传学方法分析叶维管模式形成的组成部分。叶维管格局的形成具有发展的后果超出了简单的静脉选址。在叶原基，在那里形成的静脉细胞的前体，provvascular网站似乎作为形态发生中心，调节维管和其他叶细胞类型的位置和专业化。这在功能上是有意义的，因为许多细胞类型必须与静脉合作发挥作用，需要确定的空间关系。使用正向和反向遗传方法，已经确定了这一过程的潜在组成部分，包括可能的provascular信号通路的成员。将分析他们的作用，并确定其他候选人的特点。这些是目的：1）鉴定VH1前血管受体样激酶的直接信号传导途径伙伴，其是组织血管区域的关键作用的候选者。2)确定cvp 1甾醇甲基转移酶（SMT 2）在原血管细胞定位或分化中的作用。这些过程在cvp1突变体中是有缺陷的。3)确定以粗脉突变体为代表的SAUR基因的作用。这可能代表了生长素信号和脉形成之间的直接联系。4）表征脉图案缺陷突变体鉴定的其他基因的脉图案作用。