Analysis of Vascular Pattern Formation in Leaves of Arabidopsis

0110370Nelson 将使用拟南芥中的分子遗传学方法分析叶维管束模式形成的组成部分。叶维管束模式的形成对发育的影响超出了静脉的简单定位。叶原基中形成静脉细胞前体的前维管部位似乎是调节维管细胞和其他叶细胞类型的位置和特化的形态发生中心。这具有功能意义，因为许多细胞类型必须与静脉合作发挥作用，需要明确的空间关系。使用正向和反向遗传方法，已经确定了该过程的潜在组成部分，包括前血管信号通路的可能成员。我们将分析他们的角色并确定其他候选人的特征。目标如下：1) 鉴定 VH1 前血管受体样激酶的直接信号传导途径伙伴，该激酶是在组织血管区域中发挥关键作用的候选者。 2) 确定cvp1甾醇甲基转移酶(SMT2)在原血管细胞定位或分化中的作用。这些过程在 cvp1 突变体中是有缺陷的。 3)确定粗静脉突变体所代表的SAUR基因的作用。这可能代表生长素信号和静脉形成之间的直接联系。4) 表征具有静脉图案缺陷的突变体所识别的其他基因的静脉图案作用。

0110370NelsonThe components of leaf vascular pattern formation will be analyzed, using a molecular genetic approach in Arabidopsis. The formation of the leaf vascular pattern has developmental consequences beyond the simple siting of veins. Provascular sites in the leaf primordium, where the precursors of vein cells are formed, appear to serve as morphogenetic centers that regulate the location and specialization of vascular and other leaf cell types. This makes functional sense, since many of the cell types must function in cooperation with the veins, requiring a defined spatial relationship. Using forward and reverse genetic approaches, potential components of this process have been identified, including likely members of provascular signaling pathways. Their roles will be analyzed and additional candidates will be characterized. These are the aims:1) To identify the immediate signaling pathway partners of the VH1 provascular receptor-like kinase, which is a candidate for a key role in organizing vascular regions. 2) To determine the role of the cvp1 sterol methyl transferase (SMT2) in provascular cell siting or differentiation. These processes are defective in cvp1 mutants. 3) To determine the role of the SAUR gene represented by the thick vein mutant. This may represent a direct link between auxin signals and vein formation.4) To characterize the vein patterning roles of additional genes identified by mutants with vein pattern defects.