Arabidopsis 2010: Development of Laser-Capture Microdissection for Plant Tissues

拟南芥 2010：植物组织激光捕获显微切割技术的发展

• 批准号: 0114648
• 负责人: Timothy Nelson
• 金额: $ 40万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0114648&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Development; Laser; Capture

Timothy NelsonYale UniversityIBN-0114648Development of laser-capture microdissection for plant tissuesAbstractThe technique of laser-capture microdissection (LCM) will be developed and optimized for plant tissues. This is a method originally developed for animal tissues, whereby individual cells are harvested by tacking them to a plastic film with a low-power infrared laser that can be aimed at single cells while viewing the tissue slice under a microscope. Using this method, it should be possible to recover specific cell types or developmental stages from complex tissues consisting of many cell types. Cells recovered in this manner can be analyzed with regard to gene expression profiles, protein profiles, and other properties. Based on preliminary data, it will be necessary to test a variety of tissue fixation and sectioning methods to adapt the method to plants. This project will optimize the LCM technique, using a variety of plant tissue sources and purity markers for specific cells. Detailed protocols will be provided to the biological community at the website http://plantgenomics.biology.yale.edu.

蒂莫西·纳尔逊耶​​鲁大学IBN-0114648植物组织激光捕获显微切割的开发摘要激光捕获显微切割（LCM）技术将针对植物组织进行开发和优化。这是一种最初为动物组织开发的方法，通过将单个细胞粘在塑料薄膜上，使用低功率红外激光来收获单个细胞，该激光可以瞄准单个细胞，同时在显微镜下观察组织切片。使用这种方法，应该可以从由多种细胞类型组成的复杂组织中恢复特定的细胞类型或发育阶段。可以分析以这种方式回收的细胞的基因表达谱、蛋白质谱和其他特性。根据初步数据，有必要测试各种组织固定和切片方法，以使该方法适应植物。该项目将优化 LCM 技术，使用多种植物组织来源和特定细胞的纯度标记。详细方案将在网站 http://plantgenomics.biology.yale.edu 上提供给生物界。