SGER: Evaluation of Porous Silicon Pore Size for Effective Infiltration of Biomolecules

SGER：评估多孔硅孔径对生物分子有效渗透的影响

• 批准号: 0722143
• 负责人: Sharon Weiss
• 金额: --
• 依托单位: Vanderbilt University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0722143&HistoricalAwards=false
• 关键词: SGER; Evaluation; Porous; Silicon; Pore

Sharon Weiss: SGERThe objective of this SGER proposal is to establish a quantitative relationship between porous silicon pore size and biomolecule size to enable reliable infiltration, binding and detection of target biological material using a label-free porous silicon sensing platform. Intellectual Merit: The intellectual merit of the proposed research resides in the understanding of the size relationship between biomolecules under test and the pore diameter of a porous host sensing material. The high risk challenge for porous silicon sensing is to effectively determine if the 20 nm pore size is sufficiently large to detect medically significant biomolecules. In this project the PI will carry out three specific experiments to determine the maximum biomolecule size for detection in pores with a 20 nm average diameter. (1) DNA oligos of different length (i.e., different number of base pair) will be exposed to a single layer of porous silicon. (2) Ellipsometry measurements on planar silicon wafers will be used to determine the size of the molecules used to functionalize the porous silicon.. (3) Complementary and non-complementary DNA strands will be exposed to the porous silicon. Broader impacts. The PI plans to develop outreach activities that will target underrepresented minorities through, for example, presentations to TWISTER (Tennessee Women in Science, Technology, Engineering, and Research) high school girls. The PI will also recruit undergraduate researchers, including those at nearby Fisk University (HBCU), to be involved in the proposed research.

沙龙·韦斯（Sharon Weiss）：该SGER建议的目标是建立多孔硅孔径和生物分子大小之间的定量关系，以实现可靠的浸润，结合和检测目标生物学材料，并使用无标签的多孔多孔硅传感平台。 智力优点：拟议研究的智力优点在于理解正在测试的生物分子与多孔宿主感应材料的孔径之间的尺寸关系。多孔硅传感的高风险挑战是有效确定20 nm孔径是否足够大，可以检测出医学上有意义的生物分子。在该项目中，PI将进行三个特定的实验，以确定平均直径20 nm的孔中检测的最大生物分子大小。 （1）不同长度（即不同数量的碱基对）的DNA寡素将暴露于单层多孔硅。 （2）将使用平面硅晶片上的椭圆测量测量值来确定用于使多孔硅官能化的分子的大小。（3）将暴露于多孔硅。 更广泛的影响。 PI计划通过向Twister（田纳西州科学，技术，工程和研究中的田纳西州女性）高中女生的演讲来开发针对代表性不足的少数民族的外展活动。 PI还将招募包括附近Fisk University（HBCU）在内的本科研究人员参与拟议的研究。