Porous silicon on paper-based optical biosensor for diagnostics

用于诊断的纸基光学生物传感器上的多孔硅

• 批准号: 2037673
• 负责人: Sharon Weiss
• 金额: $ 37.5万
• 依托单位: Vanderbilt University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2037673&HistoricalAwards=false
• 关键词: Porous; silicon; paper; based; optical

There is a critical need for the development of cost-effective, highly sensitive, widely deployable, rapid diagnostic testing systems that can be adapted for detecting a variety of pathogens. This type of testing system can give healthcare providers key information necessary to make educated treatment decisions, and also can facilitate epidemiological studies of disease distribution patterns. This project investigates whether incorporation of a high surface area porous nanomaterial on a paper substrate can enable a new, highly sensitive, quantitative, and reliable platform for rapid diagnostic testing. The porous nanomaterial serves as the active sensing region that produces a clear optical signal change when signature molecules are selectively captured inside the pores, while the paper substrate is a simple and cost-effective fluid delivery vehicle that enables active transport of a test solution to the porous nanomaterial. The proposed work will lead to advanced understanding of fluid flow dynamics and molecular attachment in porous nanomaterials integrated with paper as well as advanced knowledge related to interfacing nanomaterials with non-traditional substrate materials. Educationally, this program will expose students to interdisciplinary research at the intersections of optics, materials science, engineering, and chemistry. A hands-on optical biosensor demonstration kit will be developed and deployed to K-12 students through shareable videos, classroom visits, and on-campus outreach activities. The goal of this project is to demonstrate a porous silicon-on-paper optical biosensor capable of rapid, accurate, quantitative, and high sensitivity detection of protein biomarkers that will significantly advance the capabilities of rapid diagnostic testing. A comprehensive understanding of the achievable performance metrics, tolerances, and potential limitations of the porous silicon-on-paper optical biosensor platform will be attained. To accomplish this goal, key advances to realize porous silicon in a lateral flow configuration on paper and understand the fluid flow dynamics and molecular binding kinetics in such a configuration will be achieved. Specifically, this project seeks to: (1) develop a robust approach for integrating nanoscale porous films with paper-based microfluidic substrates; (2) understand molecular transport and binding kinetics in a porous film-on-paper platform as a function of the chemical and physical characteristics of the porous film and species infiltrated in the film; (3) establish the viability of using porous silicon optical thin films in a lateral flow rapid test framework; and (4) validate sensor through detection of SARS-CoV IgM and IgG antibodies.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

迫切需要开发具有成本效益，高度敏感，可部署的快速诊断测试系统，这些系统可以适应用于检测各种病原体。这种类型的测试系统可以为医疗保健提供者提供做出有根据的治疗决策所必需的关键信息，也可以促进疾病分布模式的流行病学研究。该项目研究了在纸基板上掺入高表面积多孔纳米材料是否可以使新的，高度敏感，定量和可靠的平台进行快速诊断测试。当特征分子在孔内选择性捕获时，多孔纳米材料用作有源感测区域，在毛孔内有选择地捕获签名分子时会产生明显的光学信号变化，而纸质底物是一种简单且具有成本效益的流体递送车辆，可以使测试溶液的主动运输到多孔纳米材料。提出的工作将导致对与纸张集成的多孔纳米材料的流体流动动力学和分子附着的深入了解，以及与将纳米材料与非传统底物材料连接的高级知识。在教育上，该计划将使学生在光学，材料科学，工程和化学的交叉点上进行跨学科研究。将通过可共享的视频，课堂访问和校园外展活动开发并部署了动手的光学生物传感器演示套件并将其部署给K-12学生。该项目的目的是证明能够快速，准确，定量和高灵敏度检测蛋白质生物标志物的多孔硅光学生物传感器，这些蛋白质生物标志物将显着提高快速诊断测试的能力。将获得对可实现的性能指标，公差和多孔硅光学光学生物传感器平台的潜在局限性的全面理解。为了实现这一目标，关键的进步是在纸张上的横向流配置中实现多孔硅，并了解这种构型中的流体流动动力学和分子结合动力学。具体而言，该项目试图：（1）开发一种可靠的方法，将纳米级多孔膜与纸基微流体底物相结合； （2）了解多孔胶片平台中的分子传输和结合动力学，这是膜中多孔膜和物种的化学和物理特征的函数； （3）在横向流程快速测试框架中使用多孔硅光学薄膜的生存能力； （4）通过检测SARS-COV IgM和IgG抗体来验证传感器。该奖项反映了NSF的法定任务，并且使用基金会的知识分子优点和更广泛的影响评估标准，被认为值得通过评估来获得支持。