Porous silicon on paper-based optical biosensor for diagnostics

用于诊断的纸基光学生物传感器上的多孔硅

• 批准号: 2037673
• 负责人: Sharon Weiss
• 金额: $ 37.5万
• 依托单位: Vanderbilt University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2037673&HistoricalAwards=false
• 关键词: Porous; silicon; paper; based; optical

There is a critical need for the development of cost-effective, highly sensitive, widely deployable, rapid diagnostic testing systems that can be adapted for detecting a variety of pathogens. This type of testing system can give healthcare providers key information necessary to make educated treatment decisions, and also can facilitate epidemiological studies of disease distribution patterns. This project investigates whether incorporation of a high surface area porous nanomaterial on a paper substrate can enable a new, highly sensitive, quantitative, and reliable platform for rapid diagnostic testing. The porous nanomaterial serves as the active sensing region that produces a clear optical signal change when signature molecules are selectively captured inside the pores, while the paper substrate is a simple and cost-effective fluid delivery vehicle that enables active transport of a test solution to the porous nanomaterial. The proposed work will lead to advanced understanding of fluid flow dynamics and molecular attachment in porous nanomaterials integrated with paper as well as advanced knowledge related to interfacing nanomaterials with non-traditional substrate materials. Educationally, this program will expose students to interdisciplinary research at the intersections of optics, materials science, engineering, and chemistry. A hands-on optical biosensor demonstration kit will be developed and deployed to K-12 students through shareable videos, classroom visits, and on-campus outreach activities. The goal of this project is to demonstrate a porous silicon-on-paper optical biosensor capable of rapid, accurate, quantitative, and high sensitivity detection of protein biomarkers that will significantly advance the capabilities of rapid diagnostic testing. A comprehensive understanding of the achievable performance metrics, tolerances, and potential limitations of the porous silicon-on-paper optical biosensor platform will be attained. To accomplish this goal, key advances to realize porous silicon in a lateral flow configuration on paper and understand the fluid flow dynamics and molecular binding kinetics in such a configuration will be achieved. Specifically, this project seeks to: (1) develop a robust approach for integrating nanoscale porous films with paper-based microfluidic substrates; (2) understand molecular transport and binding kinetics in a porous film-on-paper platform as a function of the chemical and physical characteristics of the porous film and species infiltrated in the film; (3) establish the viability of using porous silicon optical thin films in a lateral flow rapid test framework; and (4) validate sensor through detection of SARS-CoV IgM and IgG antibodies.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

迫切需要开发具有成本效益、高度敏感、可广泛部署的快速诊断测试系统，以适应检测各种病原体。这种类型的检测系统可以为医疗保健提供者提供必要的关键信息，以做出明智的治疗决策，也可以促进疾病分布模式的流行病学研究。该项目研究了在纸基材上加入高表面积多孔纳米材料是否可以为快速诊断测试提供一个新的、高灵敏度、定量和可靠的平台。多孔纳米材料作为主动传感区域，当特征分子被选择性地捕获到孔内时，产生清晰的光学信号变化，而纸基板是一种简单且经济高效的流体输送载体，可以将测试溶液主动输送到多孔纳米材料中。这项工作将导致对流体流动动力学和与纸张结合的多孔纳米材料中的分子附着的深入了解，以及与纳米材料与非传统衬底材料界面相关的高级知识。在教育方面，该项目将使学生接触到光学、材料科学、工程和化学交叉领域的跨学科研究。将开发一套实用的光学生物传感器演示套件，并通过可共享的视频、课堂参观和校园外展活动向K-12学生提供。该项目的目标是展示一种多孔纸上硅光学生物传感器，能够快速、准确、定量和高灵敏度地检测蛋白质生物标志物，这将大大提高快速诊断测试的能力。将全面了解可实现的性能指标，公差和多孔硅纸光学生物传感器平台的潜在限制。为了实现这一目标，将在纸上实现多孔硅的横向流动构型，并了解这种构型下的流体流动动力学和分子结合动力学。具体而言，该项目旨在：(1)开发一种将纳米级多孔膜与纸基微流体基板集成的强大方法；(2)了解多孔纸膜平台中的分子运输和结合动力学与多孔膜的化学和物理特性以及渗透在膜中的物质的关系；(3)建立了在横向流动快速测试框架中使用多孔硅光学薄膜的可行性；(4)通过检测SARS-CoV IgM和IgG抗体对传感器进行验证。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。