MRI:ID - Development of an Imaging Nonlinear Optical Ellipsometer

MRI:ID - 成像非线性光学椭偏仪的开发

• 批准号: 0722558
• 负责人: Garth Simpson
• 金额: $ 43.46万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0722558&HistoricalAwards=false
• 关键词: MRI; ID; Development; Imaging; Nonlinear

With this award from the Major Research Instrumentation program (MRI), Garth Simpson and colleagues of Purdue University will develop a SHG microscope with full polarization capability to enhance nonlinear optical Stokes ellipsometry (NOSE). The instrument will consist of an inverted optical microscope modified to allow confocal imaging in a beam-scanning configuration. The microscope will be equipped with a photoelastic modulator for rapid polarization modulation, a pair of photomultiplier tubes (PMTs) for polarization-dependent SHG detection, and a pair of PMTs for polarization dependent two photon excited fluorescence (TPEF) detection. This instrument will yield detailed polarization information and nanoscale structural information, obtained from molecular tensors and theoretical modeling. This instrument will be utilized initially to study cell wall construction (through cytoskeletal actin-myosin network dynamics) and deconstruction (enzymatic degradation of cellulosic materials for renewable energy) mechanisms. These efforts will capitalize on the chiral-specific activities of second harmonic generation (SHG), which allow for determination of absolute polar orientation and the degree of coparallel versus anti-parallel alignment within fibrous assemblies. This instrument will affect a broad range of research areas involving surfaces and interfaces.

在获得重大研究仪器计划(MRI)的这一奖项后，普渡大学的Garth Simpson和他的同事们将开发一种具有全偏振能力的倍频显微镜，以增强非线性光学斯托克斯椭圆术(NOSE)。该仪器将包括一个经过改装的倒置光学显微镜，以允许在光束扫描配置中进行共焦成像。显微镜将配备一个用于快速偏振调制的光弹性调制器，一对用于偏振相关倍频检测的光电倍增管(PMT)，以及一对用于偏振相关双光子激发荧光(TPEF)检测的光电倍增管(PMT)。该仪器将产生详细的极化信息和纳米级结构信息，这些信息是通过分子张量和理论模型获得的。该仪器最初将用于研究细胞壁的构建(通过细胞骨架肌动蛋白-肌球蛋白网络动力学)和解构(用于可再生能源的纤维材料的酶降解)机制。这些努力将利用二次谐波产生(SHG)的手性特异性活动，该活动允许确定纤维组件内的绝对极性取向和平行与反平行排列的程度。该仪器将影响到涉及表面和界面的广泛研究领域。