MRI: Track 1 Development of a Dual-Comb Fluorescence-Detected Photothermal Mid-Infrared (DC-FPTIR) Microscope

该奖项由主要研究仪器（MRI）、化学研究仪器和化学测量与成像（CMI）项目联合支持。普渡大学正在开发一种使用双梳激光源的新型荧光检测光热显微镜，以支持Garth Simpson教授及其同事Yoon Yeo， Lynn Tayler， Arezoo Ardekani和Paul Schmitt的研究。该仪器促进了纳米粒子和先进材料分析领域的研究，生物系统的基础研究，以及植物和动物分子组成的测量。该仪器使高分辨率光学显微镜与对比度产生的特定分子键。一般来说，双梳光谱学使用高频光信号来产生可以通过高速数字化检测到的射频拍模式。这些热模式调节了局部温度和相应的荧光强度。然后用荧光显微镜和依赖于局部热导率的红外荧光共定位设定的空间分辨率进行红外光谱分析。除了直接支持基础科学外，该奖项还旨在加强多个部门各级教育、研究和研究培训。该仪器还用于美国国家科学基金会支持的生物分析计量中心的活动，美国国家科学基金会本科生研究经验（REU）计划，以及新成立的青年先进制药研究所的主要推广和教育活动。该奖项旨在加强各级的研究和教育。具体来说，该仪器的开发提供了独特的能力来执行治疗纳米颗粒转化的光谱表征。该仪器能够在影响治疗材料溶解的相分离域内进行化学作图。通过使用双梳激光源，该仪器还有助于病毒成熟过程中的结构解析和成分变化。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is jointly supported by the Major Research Instrumentation (MRI), the Chemistry Research Instrumentation, and Chemical Measurement and Imaging (CMI) programs. Purdue University is developing a novel fluorescence-detected photothermal microscope using a dual-comb laser source to support the research of Professor Garth Simpson along with colleagues Yoon Yeo, Lynn Tayler, Arezoo Ardekani, and Paul Schmitt. This instrument facilitates research in the areas of nanoparticle and advanced materials analysis, fundamental studies of biological systems, and measurement of molecular composition in plants and animals. The instrument enables high-resolution optical microscopy with contrast arising from specific molecular bonds. In general, dual-comb spectroscopy uses high frequency optical signals to produce radio-frequency beat patterns that can be detected by high-speed digitization. These beat patterns modulate the local temperature and the corresponding fluorescence intensity. Infrared spectroscopy is then performed with a spatial resolution set by fluorescence microscopy and IR-fluorescence colocalization dependent on the local thermal conductivity. In addition to supporting fundamental science directly, this award targets the enhancement of educational, research, and research training at all levels in multiple departments. This developed instrument is also used to benefit activities within the NSF-supported Center for Bioanalytic Metrology, an NSF Research Experience for Undergraduates (REU) program, and major outreach and educational activities of the newly established Young Institute for Advanced Manufacturing of Pharmaceuticals. This award is aimed at enhancing research and education at all levels. Specifically, the development of this instrument offers the unique ability to perform the spectroscopy characterization of therapeutic nanoparticle transformations. The instrumentation enables chemical mapping within phase separated domains affecting dissolution in therapeutic materials. By using the dual-comb laser source, this instrument also aids in the structural elucidation and compositional changes during virus maturation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.