MRI: Track 1 Development of a Dual-Comb Fluorescence-Detected Photothermal Mid-Infrared (DC-FPTIR) Microscope

该奖项由重大研究仪器(MRI)、化学研究仪器以及化学测量和成像(CMI)计划联合支持。普渡大学正在开发一种新型的荧光探测光热显微镜，该显微镜使用双梳激光光源，以支持Garth Simpson教授及其同事Yoon Yeo、Lynn Tayler、Arezoo Ardekani和Paul Schmitt的研究。该仪器促进了纳米颗粒和先进材料分析、生物系统的基础研究以及动植物分子组成测量领域的研究。该仪器使高分辨率光学显微镜能够与特定分子键产生的对比度。通常，双梳光谱学使用高频光信号来产生可通过高速数字化检测的射频拍频图案。这些节拍模式调制局部温度和相应的荧光强度。然后用荧光显微镜设置的空间分辨率进行红外光谱分析，并根据局部导热系数进行红外-荧光共聚焦。除了直接支持基础科学外，该奖项还旨在加强多个部门各级的教育、研究和研究培训。这项开发的仪器还被用于资助NSF支持的生物分析计量中心内的活动、NSF本科生研究经验(REU)计划，以及新成立的青年药物先进制造研究所的主要外联和教育活动。该奖项旨在加强各级的研究和教育。具体地说，该仪器的开发提供了执行治疗性纳米颗粒转化的光谱表征的独特能力。该仪器能够在影响治疗材料溶出度的相分离区域内进行化学映射。通过使用双梳激光光源，该仪器还有助于病毒成熟过程中的结构阐明和成分变化。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is jointly supported by the Major Research Instrumentation (MRI), the Chemistry Research Instrumentation, and Chemical Measurement and Imaging (CMI) programs. Purdue University is developing a novel fluorescence-detected photothermal microscope using a dual-comb laser source to support the research of Professor Garth Simpson along with colleagues Yoon Yeo, Lynn Tayler, Arezoo Ardekani, and Paul Schmitt. This instrument facilitates research in the areas of nanoparticle and advanced materials analysis, fundamental studies of biological systems, and measurement of molecular composition in plants and animals. The instrument enables high-resolution optical microscopy with contrast arising from specific molecular bonds. In general, dual-comb spectroscopy uses high frequency optical signals to produce radio-frequency beat patterns that can be detected by high-speed digitization. These beat patterns modulate the local temperature and the corresponding fluorescence intensity. Infrared spectroscopy is then performed with a spatial resolution set by fluorescence microscopy and IR-fluorescence colocalization dependent on the local thermal conductivity. In addition to supporting fundamental science directly, this award targets the enhancement of educational, research, and research training at all levels in multiple departments. This developed instrument is also used to benefit activities within the NSF-supported Center for Bioanalytic Metrology, an NSF Research Experience for Undergraduates (REU) program, and major outreach and educational activities of the newly established Young Institute for Advanced Manufacturing of Pharmaceuticals. This award is aimed at enhancing research and education at all levels. Specifically, the development of this instrument offers the unique ability to perform the spectroscopy characterization of therapeutic nanoparticle transformations. The instrumentation enables chemical mapping within phase separated domains affecting dissolution in therapeutic materials. By using the dual-comb laser source, this instrument also aids in the structural elucidation and compositional changes during virus maturation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.