En-Gen: Proteomics Directed Environmental Genomics: Identifying in Situ Physiological Diversity of Cyanobacterial Nutrient Utilization in the South Atlantic Ocean

En-Gen：蛋白质组学指导的环境基因组学：鉴定南大西洋蓝藻营养物利用的原位生理多样性

• 批准号: 0723866
• 负责人: Gabrielle Rocap
• 金额: $ 59.65万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0723866&HistoricalAwards=false
• 关键词: En; Gen; Proteomics; Directed; Environmental

The unicellular marine cyanobacteria Prochlorococcus and Synechococcus account for up to 50% of primary productivity at mid latitudes in the world?s oceans. The phylogeny, physiology, and biogeography of these cyanobacteria suggest that they are comprised of several closely related ecotypes. These light adapted lineages and sublineages differ in vertical and latitudinal distributions, although the physiological adaptations that drive their distributions are not yet well understood. Comparative genomic studies of both Prochlorococcus and Synechococcus suggest that the lateral transfer of nutrient uptake and stress response genes may be driving genetic microdiversity and niche partitioning in the marine environment. Because proteins, rather than genes, determine an organism's phenotype under a defined set of conditions, proteomic approaches are ideal for in situ verification of microbial processes. This project proposes to apply a proteomic and genomic survey of the South Atlantic photic zone to explore the role of nutrient and stress response genes in shaping community structure of marine cyanobacteria. In addition to identifying actively expressed genes within diverse gene families, results from this project will help clarify relationships between nutrient dynamics and bacterioplankton community structure. Further, marine cyanobacteria provide a model system for using environmental genomic and proteomic approaches to investigate mechanisms driving niche differentiation. The project will also involve young investigators in the ocean sciences by preparing them to take advantage of genomic approaches. A post-doctoral associate and several undergraduate students will also be actively involved in the research program. Data from this project will be used to develop a computer laboratory module for a graduate course in environmental genomics taught at the University of Washington, and support from this proposal will also allow expansion of a proteomics component in the summer Microbial Oceanography course at the Bermuda Institute for Ocean Science.

单细胞海洋蓝藻原绿球藻和聚球藻占世界中纬度地区初级生产力的50%以上。的海洋。 这些蓝藻的发生，生理和生态学表明，它们是由几个密切相关的生态型。 这些光适应的谱系和亚谱系在垂直和纬度分布上不同，尽管驱动其分布的生理适应尚未得到很好的理解。 对原绿球藻和聚球藻的比较基因组研究表明，营养吸收和应激反应基因的横向转移可能是海洋环境中遗传微多样性和生态位划分的驱动力。 因为蛋白质，而不是基因，决定了一个生物体的表型在一组确定的条件下，蛋白质组学方法是理想的微生物过程的原位验证。 本研究拟应用蛋白质组学和基因组学方法对南大西洋光区进行研究，探讨营养和胁迫反应基因在塑造海洋蓝藻群落结构中的作用。 除了识别不同基因家族中活跃表达的基因外，该项目的结果将有助于澄清营养动态和浮游细菌群落结构之间的关系。 此外，海洋蓝藻提供了一个模型系统，使用环境基因组和蛋白质组学的方法来研究驱动生态位分化的机制。 该项目还将使年轻的海洋科学研究人员参与进来，使他们做好利用基因组方法的准备。一名博士后助理和几名本科生也将积极参与研究计划。 该项目的数据将用于为华盛顿大学教授的环境基因组学研究生课程开发一个计算机实验室模块，该提议的支持还将使百慕大海洋科学研究所夏季微生物海洋学课程的蛋白质组学部分得以扩大。