Stereolithography of Multi-Lumen, Multi-Material Bioactive Nerve Guidance Conduits

多腔、多材料生物活性神经引导导管的立体光刻

• 批准号: 0730750
• 负责人: Ryan Wicker
• 金额: $ 27万
• 依托单位: University of Texas at El Paso
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0730750&HistoricalAwards=false
• 关键词: Stereolithography; Multi; Lumen; Material; Bioactive

0730750WickerThe main goal of the project is to use sterolithography (SL) to manufacture unique nerve guide conduits (NGC) for repair of peripheral nerve damage. Specifically, the investigators will develop multi-lumen conduits from photopolymerizable poly ethylene glycol (PEG) hydrogels that are laden with growth factors and produced with geometry that is presently unavailable from commercial SL systems. It is hypothesized that the bioactive NGCs produced using this technology will be a significant improvement over single-lumen and other existing approaches by providing greater surface area available for supporting cells, additional pathways for axons and potential for increased nerve ''communication'' through precise placement of bioactive agents and cells. The research as four specific aims: (1) Install a SL unit within a biosafety cabinet and make specific modifications that will improve the micro-fabrication capabilities, (2) Synthsize and characterize the PEG scaffolds, (3) Fabricate bioactive scaffolds and conduct in vitro cell viability evaluations, and (4) Examine ability for PEG scaffolds to support nerve generation using a rat model. The project has the potential to lead to ''tailored'' nerve guidance channels that would advance the state of the art in this field significantly. The educational and outreach activities include support for underrepresented Hispanic students, through University of Texas at El Paso (UTEP) and the W.M. Keck Center.

0730750 wicker该项目的主要目标是利用立体光刻技术（SL）制造独特的神经引导导管（NGC），用于修复周围神经损伤。具体来说，研究人员将利用可光聚合的聚乙二醇（PEG）水凝胶开发多管腔管道，这种水凝胶含有生长因子，其几何形状目前在商业SL系统中是不可用的。据推测，使用该技术生产的生物活性NGCs将比单腔和其他现有方法有重大改进，因为它为支持细胞提供了更大的表面积，为轴突提供了额外的通路，并通过精确放置生物活性剂和细胞来增加神经“交流”的潜力。该研究有四个具体目标：(1)在生物安全柜内安装SL单元并进行特定修改，以提高微制造能力；(2)合成并表征PEG支架；(3)制备生物活性支架并进行体外细胞活力评估；(4)通过大鼠模型检测PEG支架支持神经生成的能力。该项目有可能导致“量身定制”的神经引导通道，这将大大提高该领域的技术水平。教育和推广活动包括通过德克萨斯大学埃尔帕索分校（UTEP）和W.M.凯克中心支持代表性不足的西班牙裔学生。