SGER: Carbon Nanomaterial Translocation and Transfer

SGER：碳纳米材料易位和转移

• 批准号: 0736037
• 负责人: Pu-Chun Ke
• 金额: --
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0736037&HistoricalAwards=false
• 关键词: SGER; Carbon; Nanomaterial; Translocation; Transfer

SGER: Carbon Nanomaterial Translocation and TransferOver the past four years the PI has studied the binding of RNA and single-walled carbon nanotubes (SWNTs) and first demonstrated RNA delivery by SWNTs. The PI has invented the use of lysophospholipids as a remarkably efficient method for solubilizing SWNTs.Using molecular dynamics simulations and transmission electron microscopy the PI and his team have unraveled that the binding of lysophospholipids to an SWNT as predominantly aligned along the tube axis. The PI and his collaborators have also discovered the in vivo modification of lipid-coated SWNTs by aquatic model system Daphnia magna. Towards obtaining a mechanistic understanding of nanotoxicity, the PI and his collaborators have simulated the ready uptake of fullerene C60 and the minimal translocation of fullerene derivative C60(OH)20 across a DPPC bilayer. These pioneering studies have examined carbon nanomaterials in biological and environmental systems and have facilitated the following three objectives of this proposal. 1) Understand the binding mode of fullerene C70 and phenol gallic acid for biocompatibility. 2) Detect the cellular translocation of C70-gallic acid in real time. 3) Investigate the transfer of carbon nanomaterials in the food chain.

SGER：在过去的四年里，PI研究了RNA和单壁碳纳米管（SWNTs）的结合，并首次证明了SWNTs的RNA传递。PI发明了溶血磷脂作为一种非常有效的溶解单壁碳纳米管的方法，利用分子动力学模拟和透射电子显微镜，PI和他的团队揭示了溶血磷脂与单壁碳纳米管的结合主要是沿着管轴沿着。PI和他的合作者还发现了水生模型系统Daphnia magna对脂质涂层SWNT的体内修饰。为了获得对纳米毒性的机械理解，PI和他的合作者模拟了富勒烯C60的快速吸收和富勒烯衍生物C60（OH）20穿过DPPC双层的最小易位。这些开创性的研究审查了生物和环境系统中的碳纳米材料，并促进了本提案的以下三个目标。 1)了解富勒烯C70与苯酚没食子酸的结合方式，以获得生物相容性。 2)真实的实时检测C70-没食子酸的细胞转位。 3)研究碳纳米材料在食物链中的转移。