SGER: Exploratory studies of marine microbes using nanoSIMS

这项探索性研究小额赠款为博士后研究和培训提供支持，使用纳米sims或纳米二级离子质谱法。纳米SIMS是一种先进的方法，用于分析50纳米分辨率下生物材料中金属和同位素组成的分布。因此，人们可以检查细胞内和细胞间的相关金属，如铁和钼的分布以及许多元素的同位素组成。这种方法提供了揭示这些元素的摄取和代谢的复杂性的手段。该研究人员与劳伦斯利弗莫尔国家实验室（LLNL）建立了合作关系，探索纳米sims在海洋微生物研究中的应用。虽然系统上的访问时间对许多竞争用户来说是一个溢价，但在2007- 2008年，研究者可以获得波束时间。这个机会使博士后能够与LLNL的nanoSIMS小组一起进行与海洋生态系统相关的生物地球化学和生物学导向领域的探索性研究。其中包括海洋浮游植物和细菌中重要的生物化学金属如铁、钼、钴和锌的细胞分布，作为其细胞代谢和生理的指标；稳定同位素如13C和15N的精细尺度内和细胞间分布，作为细胞功能的指标；以及在富集示踪剂研究中，以辨别细胞内和细胞间的代谢和代谢物交换。相关的过程是固氮和硝酸盐吸收，维生素B12代谢和碳酸酐酶活性。博士后（Sophie Bonnet）将与南加州大学的几位研究人员（Capone, Sanudo-Wilhelmy, Moffett, Hutchins, Nealson, Berelson）以及利弗莫尔实验室的科学家和技术人员在nanoSIMS设施进行实验。

This Small Grant for Exploratory Research provides support for post doctoral research and training using NanoSIMS or nano scale secondary ion mass spectrometry. Nano SIMS is a state of the art approach for analyzing the distribution of metals and isotopic composition in biological materials at 50 nm resolution. Hence, one may examine the intracellular and intercellular distributions of relevant metals such as iron and molybdenum as well as the isotopic composition of many elements.This approach provides the means to reveal the intricacies of uptake and metabolism of these elements. The investigator has established a collaboration with Lawrence Livermore National Laboratory (LLNL) to explore the application of nanoSIMS for studies of marine microorganisms. While access time on the system is at a premium with many competing users, beam time has been made available to the investigator in 2007- 2008. This opportunity allows the post doc to undertake exploratory research with the nanoSIMS group at LLNL into biogeochemically and biologically oriented areas of relevance to marine ecosystems. These include the cellular distribution of biochemically important metals such Fe, Mo, Co and Zn in marine phytoplankton and bacteria as indicators of their cellular metabolism and physiology, the fine scale intra- and intercellular distribution of stable isotopes such as 13C and 15N as indicators of cellular function, and in enriched tracer studies in order to discern metabolism and metabolite exchange within and among cells.Processes of relevance are nitrogen fixation and nitrate uptake, vitamin B12 metabolism and carbonic anhydrase activity.The postdoctoral associate (Sophie Bonnet) will interact with several researchers at USC (Capone, Sanudo-Wilhelmy, Moffett, Hutchins, Nealson, Berelson) as well as the scientists and technicians at the Livermore Lab in undertaking experiments at the nanoSIMS facility.