Dissertation Research: FUNCTIONAL CONSEQUENCES OF ADAPTIVE VARIATION AT CHROMATIN REMODELING GENES

论文研究：染色质重塑基因适应性变异的功能后果

• 批准号: 0806205
• 负责人: David Begun
• 金额: $ 1.2万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0806205&HistoricalAwards=false
• 关键词: Dissertation; Research; FUNCTIONAL; CONSEQUENCES; ADAPTIVE

The dynamic nature of chromatin (i.e., DNA complexed with its protein packaging) is associated with dynamic gene expression across the genome. Chromatin dynamics are environment-sensitive, yet the contribution of chromatin remodelers (proteins that modulate these dynamics) to adaptation to novel environmental conditions is unknown. Preliminary work described several chromatin remodeling genes that bear the signature of local adaptation in the commonly studied fruit fly Drosophila melanogaster. This project will characterize the roles of the alternative chromatin remodeling gene variants by measuring patterns of gene expression, and to describe natural variation in chromatin state (static or dynamic) in response to environmental variation. These questions will be addressed using technology that enables gene expression and protein-DNA interactions to be studied within entire genomes.This research will shed light on a novel role for chromatin remodeling proteins. It will also describe novel gene variants that may reveal novel gene function. Recent studies demonstrate a link between mutations in chromatin remodeling and aberrant gene expression profiles associated with many human diseases, including cancer. Natural variation associated with environmental variation, specifically environmental stress, documented in Drosophila may shed light on human disease variation associated with chromatin biology.

染色质的动态性质（即，与其蛋白质包装复合的DNA）与整个基因组的动态基因表达相关。染色质动力学是环境敏感的，但染色质重塑（调节这些动力学的蛋白质）对适应新的环境条件的贡献是未知的。初步的工作描述了几个染色质重塑基因，承担当地的适应签名，在通常研究的果蝇。该项目将通过测量基因表达模式来表征替代染色质重塑基因变体的作用，并描述响应环境变化的染色质状态（静态或动态）的自然变化。这些问题将通过在整个基因组中研究基因表达和蛋白质-DNA相互作用的技术来解决，这项研究将揭示染色质重塑蛋白的新作用。它还将描述可能揭示新基因功能的新基因变体。最近的研究表明，染色质重塑和异常基因表达谱与许多人类疾病，包括癌症相关的突变之间的联系。果蝇中记录的与环境变化相关的自然变异，特别是环境应激，可能揭示与染色质生物学相关的人类疾病变异。