Probing Protein-Salt Interactions with Microfluidics and Nonlinear Optics

利用微流体和非线性光学探测蛋白质-盐相互作用

• 批准号: 0809854
• 负责人: Paul Cremer
• 金额: $ 41.6万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0809854&HistoricalAwards=false
• 关键词: Probing; Protein; Salt; Interactions; Microfluidics

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. Paul Cremer of Texas A&M University. Prof. Cremer and his students will study the fundamental mechanisms of the interactions of salts with model polypeptide systems. The study will reveal the profound effects that salts have on the physical properties of proteins. These interactions play crucial role in many processes including protein folding, enzyme turnover and supramolecular ordering. The study will involve the use of a wide variety of analysis tools including vibrational sum frequency spectroscopy (VSFS) and a novel temperature gradient microfluidic device which was developed by Prof. Cremer and his students. The study will provide an excellent educational opportunity for graduate students and postdoctoral trainees to carry out a fundamental protein dynamics study using state of the art analytical techniques. The study will be applicable to a wide variety of problems ranging from polymer physics and medicine to colloid chemistry and protein isolation.

化学系的分析和表面化学（ASC）项目将支持德克萨斯农工大学Paul Cremer教授的研究项目。Cremer教授和他的学生将研究盐与模型多肽系统相互作用的基本机制。这项研究将揭示盐对蛋白质物理性质的深远影响。这些相互作用在蛋白质折叠、酶周转和超分子排序等过程中起着至关重要的作用。该研究将涉及使用多种分析工具，包括振动和频率光谱（VSFS）和由Cremer教授和他的学生开发的新型温度梯度微流控装置。该研究将为研究生和博士后学员提供一个极好的教育机会，使用最先进的分析技术进行基本的蛋白质动力学研究。该研究将适用于从聚合物物理和医学到胶体化学和蛋白质分离的各种问题。