CAREER: The Behavior of Proteins at the Solid-Liquid Interface Monitored in situ with Infrared-Visible Sum Frequency Generation: Investigating the Role of Interfacial Water

职业：利用红外-可见光和频生成原位监测固-液界面蛋白质的行为：研究界面水的作用

• 批准号: 0094332
• 负责人: Paul Cremer
• 金额: $ 48.2万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0094332&HistoricalAwards=false
• 关键词: CAREER; Behavior; Proteins; Solid; Liquid

This CAREER project addresses the molecular level structure of water at the liquid solid interface in the presence of adsorbed protein molecules. Professor Paul Cremer and his coworkers at Texas A&M, with the support of the Analytical and Surface Chemistry Program, will examine the interactions of water, protein molecules, and solid silica surfaces. An understanding of these types of systems is important to a variety of applications, including biocompatibility, biofouling in the food and pharmaceutical industries, and in the development of biosensors and catalysts. Infrared-visible sum frequency generation methods will be used to probe the structure of substrate associated water in the presence of adsorbed protein. Time resolved studies will be implemented to follow structural changes upon adsorption of protein molecules at the solid surface. Educational aspects of this project include the development of a bio-physical chemistry course for upper level undergraduates, and the implementation of undergraduate research projects in the field of protein surface interactions. The interaction of protein molecules with surfaces is important in the development of biosensors, in enhancing biocompatibility for medical implants, and for understanding the process of biofouling in food and pharmaceutical process machinery. The structure of adsorbed water in these systems provides the detailed information that can be used to design surfaces with desired protein adsorption properties. This CAREER project uses state of the art surface spectroscopic methods to probe this structural question. Integration of this basic research with educational goals in the training of bio-physical scientists and in the development of course materials in this area is the focus of the educational aspect of this project.

这个CAREER项目解决了在存在吸附蛋白质分子的情况下，水在液固界面的分子水平结构。 教授保罗克雷默和他的同事在得克萨斯州A M，与分析和表面化学计划的支持下，将检查水，蛋白质分子和固体二氧化硅表面的相互作用。 了解这些类型的系统是很重要的各种应用，包括生物相容性，在食品和制药行业的生物污垢，并在生物传感器和催化剂的发展。 可见光和频产生方法将用于探测在吸附蛋白存在下底物缔合水的结构。 将实施时间分辨研究，以跟踪蛋白质分子在固体表面吸附后的结构变化。 该项目的教育方面包括为高水平本科生开发生物物理化学课程，以及在蛋白质表面相互作用领域实施本科生研究项目。蛋白质分子与表面的相互作用在生物传感器的开发、增强医疗植入物的生物相容性以及理解食品和制药加工机械中的生物污染过程中是重要的。 在这些系统中的吸附水的结构提供了详细的信息，可用于设计具有所需的蛋白质吸附性能的表面。 这个CAREER项目使用最先进的表面光谱方法来探测这个结构问题。 将这一基础研究与生物物理科学家培训和这一领域课程教材编制的教育目标相结合，是该项目教育方面的重点。