SBIR Phase I: Electron Capture Dissociation for Radiofrequency Ion Trap MS

SBIR 第一阶段：射频离子阱 MS 的电子捕获解离

• 批准号: 0810794
• 负责人: Jack Syage
• 金额: --
• 依托单位: SYAGEN TECHNOLOGY INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0810794&HistoricalAwards=false
• 关键词: SBIR; Phase; Electron; Capture; Dissociation

This Small Business Innovation Research Phase I project will test the feasibility of an electron capture dissociation (ECD) method for radio-frequency (RF) ion trap mass spectrometers. ECD has emerged as a powerful means to fragment peptide and protein bonds in a manner that can reveal details of post-translational modifications (PTMs). ECD is difficult to implement in RF driven mass analyzers, such as quadrupole and linear ion traps, because the RF fields drive electrons to excess energy and out of the trap. This research demonstrates the feasibility for an ECD method for ion traps using a method of gating the RF off for short periods of time, while introducing low-energy electrons to achieve ECD. The broader impacts of this research are in healthcare and disease control. Studying the entire complement of proteins expressed by a particular cell, organism or tissue at a given time and under a specific set of environmental conditions will require developing new automated and high-speed methods for analyzing the protein content of cell lines. The proposed ECD method for ion traps will lead to accurate identification of protein structure including PTM sites. This diagnostic capability has the potential to provide systematic searches for biomarkers. Studies of cancer cell lines can reveal signatures of cancerous cells that can serve as biomarkers for actual diagnosis. The economic impacts in terms of increased productivity and decreased burden on the health care system are substantial.

该小型企业创新研究第一阶段项目将测试射频 (RF) 离子阱质谱仪电子捕获解离 (ECD) 方法的可行性。 ECD 已成为一种强大的肽和蛋白质键片段化方法，可以揭示翻译后修饰 (PTM) 的详细信息。 ECD 很难在 RF 驱动的质量分析仪（例如四极杆和线性离子阱）中实现，因为 RF 场将电子驱动到多余的能量并离开陷阱。这项研究证明了离子陷阱 ECD 方法的可行性，该方法使用短时间关闭 RF 的方法，同时引入低能电子来实现 ECD。这项研究的更广泛影响是在医疗保健和疾病控制方面。研究特定细胞、生物体或组织在给定时间和特定环境条件下表达的全部蛋白质需要开发新的自动化和高速方法来分析细胞系的蛋白质含量。所提出的离子阱 ECD 方法将能够准确识别包括 PTM 位点在内的蛋白质结构。这种诊断能力有可能提供生物标志物的系统搜索。对癌细胞系的研究可以揭示癌细胞的特征，这些特征可以作为实际诊断的生物标志物。提高生产力和减轻医疗保健系统负担方面的经济影响是巨大的。