SBIR Phase I: High Speed MS^n (Molecular Sequencing) Sequencing and Structure Analysis

SBIR 第一阶段：高速 MS^n（分子测序）测序和结构分析

• 批准号: 0320149
• 负责人: Jack Syage
• 金额: $ 9.99万
• 依托单位: SYAGEN TECHNOLOGY INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0320149&HistoricalAwards=false
• 关键词: SBIR; Phase; Speed; MS; Molecular

This Small Business Innovation Research (SBIR) Phase I project proposes to test the feasibility of a new method for achieving high-speed sequencing and structure analysis of drug and biological molecules. The proposed work is expected to demonstrate about 10x faster analysis speeds using quadrupole ion trap, time-of-flight (QitTOF) mass spectrometer (MS). The main test will be new MSn sequencing routines that will benefit conventional ion trap MS as well as QitTOF MS. The goal is to achieve sequencing and structure information for fast chromatographic methods such as capillary liquid chromatography (LC) and capillary electrophoresis (CE). In this work we will demonstrate these capabilities for protein digest mixtures of peptides using a CE / QitTOF MS. Furthermore, the TOF analyzer has the potential to measure accurate masses for elemental composition. This capability will be tested for fragment loss accurate mass analysis.The commercial application of this project will be in the area of proteomics. There is a tremendous need to develop automated methods of protein analysis and peptide analysis of cell lines to better understand global biological function for improved drug therapy and early detection of disease, such as cancer.

该小型企业创新研究（SBIR）第一阶段项目旨在测试一种实现药物和生物分子高速测序和结构分析的新方法的可行性。预计拟议的工作将证明使用四极离子阱，飞行时间（QitTOF）质谱仪（MS）的分析速度快约10倍。主要测试将是新的MSn测序程序，这将有利于传统的离子阱MS以及QitTOF MS。目标是实现快速色谱方法，如毛细管液相色谱（LC）和毛细管电泳（CE）的测序和结构信息。在这项工作中，我们将使用CE / QitTOF MS证明这些能力的肽的蛋白质消化混合物。此外，TOF分析仪有可能测量元素组成的准确质量。该项目的商业应用将在蛋白质组学领域进行。非常需要开发细胞系的蛋白质分析和肽分析的自动化方法，以更好地了解整体生物学功能，用于改进药物治疗和早期检测疾病，如癌症。