Regulation and Function of Membrane Lipid Hydrolysis in Lipid-Based Signaling of Plants

植物脂质信号传导中膜脂质水解的调节和功能

• 批准号: 0818740
• 负责人: Xuemin Wang
• 金额: $ 42万
• 依托单位: University of Missouri-Saint Louis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0818740&HistoricalAwards=false
• 关键词: Regulation; Function; Membrane; Lipid; Hydrolysis

Regulation and Function of Membrane Lipid Hydrolysis in Lipid-Mediated Signaling in PlantsGrant proposal number: IOS-0818740PI: Xuemin WangPROJECT SUMMARY Cell membranes are rich sources for producing bioactive compounds that affect plant growth, development, and stress responses. This project investigates the molecular mechanism by which one class membrane lipid-hydrolyzing enzyme phospholipase D (PLD) and its product phosphatidic acid (PA) mediate plant survival, growth, and biomass accumulation. The central hypothesis is that specific PLDs and PAs mediate plant growth by interacting with proteins in carbon metabolism and translation regulation. This project will characterize the interaction of PLD/PA with one group of enzymes in carbon metabolism and another in translation regulation. The PA/PLD-effector interactions will be characterized using different approaches, including quantitative biophysical analysis and open-ended profiling of proteins and lipids in the regulatory complexes. The intellectual merits of the research are to discover direct connections of membrane-based signaling with energy metabolism and growth control and to identify novel regulatory processes in plants with potential to improve plant production. The proposed activity will have broader impacts on science, education, and society. The proposed work has the potential to transform current knowledge on the molecular and biochemical mechanism by which cells integrate stress and growth cues for optimizing plant production. It will provide opportunities to train students and researchers in emerging, under-explored disciplines in plant biology. It will serve as a platform to broaden participation of underrepresented groups in research. The information will be disseminated to enhance science and education through lectures and seminars, classroom teaching, scientific meetings, and publications. The knowledge gained may be applicable to developing crop plants with enhanced stress tolerance and productivity.

植物脂质介导信号传导中膜脂质水解的调节和功能资助申请号：IOS-0818740 PI：王学敏项目摘要细胞膜是产生影响植物生长、发育和应激反应的生物活性化合物的丰富来源。本项目研究一类膜脂水解酶磷脂酶D（PLD）及其产物磷脂酸（PA）介导植物存活、生长和生物量积累的分子机制。中心假设是，特定的PLD和PAs介导的植物生长通过与蛋白质的碳代谢和翻译调控。 本项目将表征PLD/PA与碳代谢中的一组酶和翻译调节中的另一组酶的相互作用。PA/PLD-效应器相互作用将使用不同的方法进行表征，包括定量生物物理分析和调节复合物中蛋白质和脂质的开放式分析。该研究的智力价值在于发现基于膜的信号传导与能量代谢和生长控制的直接联系，并确定植物中具有提高植物产量潜力的新调控过程。 拟议的活动将对科学、教育和社会产生更广泛的影响。 拟议的工作有可能改变目前的分子和生物化学机制的知识，细胞整合应力和生长的线索，优化植物生产。它将提供机会，培训学生和研究人员在新兴的，未开发的植物生物学学科。 它将作为一个平台，扩大代表性不足的群体对研究的参与。 将通过讲座和研讨会、课堂教学、科学会议和出版物传播这些信息，以加强科学和教育。所获得的知识可能适用于开发具有增强的胁迫耐受性和生产力的作物植物。