Collaborative Research: Metabolomic Profiling and Functions of Oxidized Membrane Lipids in Plant Stress Responses

合作研究：氧化膜脂质在植物胁迫反应中的代谢组学分析和功能

• 批准号: 0920681
• 负责人: Xuemin Wang
• 金额: $ 41.1万
• 依托单位: University of Missouri-Saint Louis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920681&HistoricalAwards=false
• 关键词: Collaborative; Research; Metabolomic; Profiling; Functions

Metabolomic profiling and functions of oxidized membrane lipids in plant stress responses Ruth Welti and Gary L. Gadbury, Kansas State University Jyoti Shah, University of North Texas Xuemin (Sam) Wang, University of Missouri, St. Louis and Danforth Plant Science Center Increasing evidence indicates that environmental stresses, such as freezing, high salinity, and pathogen infection, lead to oxidative modification of plant membrane lipids to produce "ox-lipids". In contrast to oxylipins, such as jasmonic acid and its derivatives, whose significance in plant growth and defense against stress has been well documented, little is known about the functions of ox-lipids in plants. Ox-lipids may function as mediators signaling stress responses, they may represent damage that could serve as a protective buffer against oxidative damage elsewhere in the cell, or they may be long-term modifications that might function as stress "memory". Thus, ox-lipids have the potential to be essential mediators of plant response to the environment. The goals of the research project are to understand the role of ox-lipids in plant responses to biotic and abiotic stresses and to determine the function of members of two enzyme families, lipoxygenases and acyl hydrolases, which are likely to play important roles in the metabolism of oxidized lipids. The project will test the hypotheses that patterns of ox-lipids are fingerprints of individual stresses and that production and/or removal of specific ox-lipids by lipoxygenases and acyl hydrolases contributes to plant adaptation to stress. Under freezing and high salinity stress (abiotic stress) and infection by a fungal pathogen, Botrytis cinerea, and a bacterial pathogen, Pseudomonas syringae (biotic stresses), the stress-response phenotype and production of ox-lipids by wild-type plants and lipoxygenase- and acyl hydrolase-deficient mutant plants will be documented. The data will shed light on the roles of lipoxygenases and acyl hydrolases in stress responses and in production of specific ox-lipid patterns. Analysis of the stress-phenotype and ox-lipid profiles will lead to identification of ox-lipids that are candidates for mediating plant stress responses. The function of candidate lipid mediators will be tested by lipid analysis and phenotypic analysis of plants overexpressing enzymes that produce the candidate lipids and by supplementing mutant and wild-type plants with the putative mediators. The results have the potential to fill critical gaps in understanding of how lipid metabolic enzymes, cellular lipids, and their metabolites interact to influence plant performance. Broader Impacts: Carrying out the proposed work will provide training for multiple students and postdoctoral trainees at four institutions and bring current knowledge of metabolic profiling, functional genomics, and stress biology to the classroom. It will broaden the participation of underrepresented groups in research through the McNair Program at the University of North Texas, the Summer Undergraduate Research Opportunity Program at Kansas State University, the Des Lee Collaborative Scholarships at the University of Missouri, and the Danforth Plant Science Center NSF REU-Site program, which has achieved over 30% participation by underrepresented minority groups in the past several years. It will involve high school students in the research through the Texas Academy of Mathematics and Science at University of North Texas and through the Students and Teachers as Research Scientists (STARS) program in St. Louis. Organization of mass spectral data on plant lipids, and particularly on stress-induced lipids, into a web-accessible database will provide a foundation for further investigation of the structure and function of lipids, and particularly novel lipids, and will facilitate integration of lipidomics data with other metabolomics and functional genomics data. Analytical capabilities developed in this work will become enabling technologies available to researchers worldwide via the Kansas Lipidomics Research Center. This work also will provide insight into the identity of metabolic steps with potential to enhance stress tolerance in plants and improve agricultural productivity and quality.

Ruth Welti和Gary L. Gadbury，堪萨斯州立大学Jyoti Shah，北德克萨斯大学Xuemin (Sam) Wang，密苏里大学圣路易斯分校和Danforth植物科学中心越来越多的证据表明，环境胁迫，如冰冻、高盐度和病原体感染，导致植物膜脂氧化修饰产生“ox-lipids”。与氧脂类（如茉莉酸及其衍生物）在植物生长和抗胁迫中的重要作用相比，氧脂类在植物中的功能知之甚少。ox -脂质可能作为应激反应的介质，它们可能代表损伤，可以作为细胞其他地方氧化损伤的保护性缓冲，或者它们可能是长期的修饰，可能作为应激“记忆”。因此，氧脂具有成为植物对环境反应的重要介质的潜力。该研究项目的目标是了解氧脂在植物对生物和非生物胁迫的反应中的作用，并确定两个酶家族成员的功能，脂氧合酶和酰基水解酶，这两个酶家族可能在氧化脂的代谢中起重要作用。该项目将测试以下假设：ox-脂的模式是个体胁迫的指纹，以及通过脂氧合酶和酰基水解酶产生和/或去除特定的ox-脂有助于植物适应胁迫。在冰冻和高盐度胁迫（非生物胁迫）以及真菌病原体灰葡萄孢菌和细菌病原体丁香假单胞菌（生物胁迫）的感染下，将记录野生型植物和缺乏脂氧合酶和酰基水解酶突变植物的应激反应表型和ox-脂的产生。这些数据将阐明脂氧合酶和酰基水解酶在应激反应和特定ox-脂质模式产生中的作用。对胁迫表型和ox-脂质谱的分析将有助于鉴定介导植物胁迫反应的候选ox-脂质。候选脂质介质的功能将通过脂质分析和过表达产生候选脂质的酶的植物的表型分析，以及通过向突变型和野生型植物补充假定的介质来测试。这些结果有可能填补在了解脂质代谢酶、细胞脂质及其代谢物如何相互作用影响植物生产性能方面的关键空白。更广泛的影响：开展拟议的工作将为四所机构的多名学生和博士后培训生提供培训，并将代谢谱、功能基因组学和压力生物学的最新知识带入课堂。它将通过北德克萨斯大学的麦克奈尔项目、堪萨斯州立大学的暑期本科生研究机会项目、密苏里大学的德斯·李合作奖学金和丹佛斯植物科学中心NSF REU-Site项目扩大代表性不足群体在研究中的参与，该项目在过去几年中已使代表性不足的少数群体的参与率超过30%。它将通过北德克萨斯大学的德克萨斯数学与科学学院以及圣路易斯的学生和教师作为研究科学家（STARS）项目让高中生参与研究。将植物脂质的质谱数据，特别是胁迫诱导的脂质数据组织到一个可访问的网络数据库中，将为进一步研究脂质，特别是新型脂质的结构和功能提供基础，并将促进脂质组学数据与其他代谢组学和功能基因组学数据的整合。在这项工作中开发的分析能力将通过堪萨斯脂质组学研究中心成为全球研究人员可用的技术。这项工作还将深入了解具有增强植物抗逆性和提高农业生产力和质量潜力的代谢步骤的特性。