Metabolomic Profiling of Lipids and Their Compositional Dynamics in Plant Stress Responses

该项目汇集了具有互补专业知识的研究人员，以发展代谢组学能力，定量分析植物中的脂质分子种类及其变化。 该项目的长期目标是了解细胞脂质及其代谢产物如何相互作用以产生和维持复杂的细胞膜并调节细胞功能。 该项目的目标是双重的：一个是开发一种综合能力，使用基于质谱的通用平台来全面分析细胞脂质种类。 另一个是利用分析能力，以确定神秘的，马铃薯糖蛋白样酰基水解酶和几个假定的脂肪酶参与植物胁迫反应的代谢功能。本申请的具体目的是：（1）分析和定量调节脂质：游离脂肪酸、选择的脂肪酸衍生物、溶解脂质种类和磷酸肌醇;（2）分析和定量中性甘油酯：二酰基甘油和三酰基甘油;（3）分析和定量鞘脂分子种类;（4）发现新的脂质种类和代谢物;（5）使用谱分析能力来确定推定的脂解酶的代谢功能：马铃薯糖蛋白样蛋白、RL-PLA、AAM 10310、AtSABP 2、PRLIP 1、PAD 4和EDS 1。 电喷雾串联质谱（ESI-MS/MS）是一种灵敏、高效的脂质分析方法，有可能实现细胞脂质的全面表征。这种能力的发展将大大有助于新兴的综合研究战略和代谢组学。脂质分析将提供一个强有力的战略，以解决涉及脂质的功能的生物学问题。 功能研究将深入了解拟南芥脂质水解酶的体内底物和产物、酶被激活的条件以及活性的空间分布。该项目将产生更广泛的影响。除了通过研究活动培训研究生外，该项目还将把代谢谱和功能基因组学的现有知识带到课堂上。它还将为扩大植物科学中代表性不足的群体的参与提供机会。 堪萨斯脂质组学研究中心提供的能力和服务对许多研究人员来说都很重要。该项目将确定介导植物生长和胁迫反应的重要代谢和调节步骤。这些步骤的操作可导致产生具有增强的胁迫耐受性和提高的产品质量和/或生产力的作物植物。

This project assembles researchers with complementary expertise to develop the metabolomic capacity to quantitatively profile lipid molecular species and their changes in plants. The long-term goal of this project is to understand how cellular lipids and their metabolites interact to produce and maintain complex cell membranes and to regulate cell functions. The goal of this project is two-fold: One is to develop a comprehensive capacity to fully profile cellular lipid species, using a mass spectrometry-based, common platform. The other is to use the profiling capability to determine the metabolic function of enigmatic, patatin-like acyl hydrolases and several putative lipases involved in plant stress responses. The specific objectives of this application are to: (1) profile and quantify regulatory lipids: free fatty acids, selected fatty acid derivatives, lysolipid species, and phosphoinositides; (2) profile and quantify neutral glycerides: diacylglycerol and triacylglycerol; (3) profile and quantify sphingolipid molecular species; (4) discover new lipid species and metabolites; (5) use the profiling capability to determine the metabolic functions of putative lipolytic enzymes: the patatin-like proteins, RL-PLA, AAM10310, AtSABP2, PRLIP1, PAD4, and EDS1. Sensitive and efficient lipid profiling by electrospray ionization tandem mass spectrometry (ESI-MS/MS) has the potential to achieve full characterization of cellular lipids. The development of this capability will contribute greatly to the emerging, comprehensive research strategy, and metabolomics. Lipid profiling will provide a powerful strategy to address biological questions that involve the function of lipids. The functional studies will produce insights into in vivo substrates and products of Arabidopsis lipid-hydrolyzing enzymes, the conditions under which the enzymes are activated, and the spatial distribution of the activity.The project will have broader impacts. In addition to training graduate students through research activities, this project will bring current knowledge of metabolic profiling and functional genomics to the classroom. It will also provide an opportunity to broaden participation of underrepresented groups in the plant sciences. The capability and service provided by the Kansas Lipidomics Research Center will be important to many researchers. The project will identify important metabolic and regulatory steps mediating plant growth and stress responses. Manipulation of these steps may lead to production of crop plants with enhanced stress tolerance and increased product quality and/or productivity.