MRI: Rapid Image Acquisition of Dynamic Arabidopsis Cells and for High-Throughput Genetic Screens

一笔赠款已授予位于查佩尔山的北卡罗来纳州大学，由艾伦·M·卡耐基博士监督。Jones的题为“Rapid Image Acquisition of Dynamic Arabidopsis Cells and For High-throughput Genetic Screens”的论文中描述了用于获取和维护专用显微镜的方法。从拟南芥基因组测序开始，植物科学取得了巨大的进步，将研究推向了下一个更高的研究层面，在高空间分辨率下研究细胞蛋白质如何以及何时随着时间的推移相互作用已成为需求的新标准。同时，对复杂成像仪器的需求和需要都增加了。然而，在植物细胞中成像荧光标记的蛋白质面临着几个可克服的挑战。一个是植物细胞的动态性质，由于细胞质流动（快速细胞运动），其发生速度大于标准共聚焦显微镜（这是一种专用显微镜，使人们能够可视化各种活细胞中的荧光修饰蛋白质）的标准捕获速度。因此，为了捕捉植物细胞内的瞬时蛋白质-蛋白质相互作用，需要一种专门的共聚焦显微镜，该显微镜能够在短时间内几乎同时通过细胞进行快速采集。这些数据的重建将用于产生植物蛋白质-蛋白质相互作用随时间推移在体内的三维视图。该基金将使8位高产植物科学家在基础科学领域做出更大、更快的贡献，从而提高作物产量、植物抗病性和生物燃料生产。更广泛的影响将发生在课堂、公共场所和研究实验室。共聚焦显微镜的使用将介绍给高年级学生在一个激烈的，动手细胞生物学实验室。公众将通过PI的教育展示和演示了解快速成像的力量。植物科学组每年共培养本科生20名，博士预科生15名，博士后30名。所要求的仪器还将满足校园内许多其他实验室的共焦成像需求。这是第一个这样的校园里，只有第二个像它在北卡罗来纳州。植物科学组以外的许多科学家将大大受益于这种专用显微镜。

A grant has been awarded to The University of North Carolina at Chapel Hill (UNC) under the supervision of Dr. Alan M. Jones entitled "Rapid Image Acquisition of Dynamic Arabidopsis Cells and For High-throughput Genetic Screens" for the purpose of acquiring and maintaining a specialized microscope. The enormous progress made in the plant sciences from sequencing the Arabidopsis genome has propelled research to the next higher research plane, where examining how and when cellular proteins interact over time at a high spatial resolution has become the new standard in demand. Both the demand and the need for sophisticated imaging instrumentation have increased, pari passu. However, imaging fluorescently-tagged proteins in plant cells faces several surmountable challenges. One is the dynamic nature of plant cells due to cytoplasmic streaming (rapid cellular movement) that occurs with speeds greater than the speed of standard capture by a standard confocal microscope (which is a specialized microscope that enables one to visualize fluorescently-modified proteins in all kinds of live cells). Therefore, to capture transient protein-protein interactions within plant cells requires a specialized confocal microscope that enables rapid acquisitions nearly simultaneously through the cell, over a short time scale. Reconstitution of this data will be used to produce a 3-dimensional view of plant protein-protein interactions in vivo over time. This grant will enable 8 highly-productive plant scientists at UNC to make significant and faster contributions to fundamental science leading to increased crop production, plant disease resistance, and biofuels production.The broader impact will occur in the classroom, in public, and in the research labs. The use of a confocal microscope will be introduced to upperclassmen in an intense, hands-on cell biology laboratory. The public will become aware of the power of rapid imaging through an educational display and presentations by the PIs. The plant science group at UNC collectively trains 20 undergraduates, 15 predoctoral, and 30 postdoctoral students each year. The requested instrument will also serve the confocal imaging needs of many additional labs on campus. It is the first of its kind on the UNC campus and only the second like it in the state of North Carolina. Many scientists beyond the plant science group will greatly benefit from this specialized microscope.