MRI: Acquisition of a 200 kV Electron Tomography Microscope for Quantitative High Resolution Imaging of Cells in 3 Dimensions

MRI：购买 200 kV 电子断层扫描显微镜，对细胞进行 3 维定量高分辨率成像

• 批准号: 0821432
• 负责人: Pietro De Camilli
• 金额: $ 71.8万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821432&HistoricalAwards=false
• 关键词: MRI; Acquisition; 200; kV; Electron

A grant has been awarded to Yale University School of Medicine to acquire an electron tomography microscope for quantitative high-resolution imaging of cells and nanomaterials in three dimensions (3D). With its impressive high resolution (~5 nanometers) the new microscope will make it possible to visualize thick biological sections and other objects volumetrically, and will be used by more than 17 laboratories from 11 Yale departments. Specific focus areas will include high-resolution imaging of neurons and synapses, vesicular traffic, cytoskeleton, and nanomaterials, as well as the development of new methods of image analysis.The grant will have a broad impact in the Yale scientific community and beyond. It will help train students and postdoctoral fellows in powerful new imaging techniques, forge new collaborations across discipline boundaries, bring scientists from diverse backgrounds together, create new partnerships with the private sector, develop methodology to analyze and mine tomography datasets, and provide a strong program for education and outreach to the local community (via links to local high schools and Yale ?STARS?, SURF, Biostep programs). In all of these ways, the award will support the scientific, educational, and diversity aims of Yale University and the National Science Foundation.

耶鲁大学医学院获得了一笔拨款，用于购买一台电子断层扫描显微镜，用于对细胞和纳米材料进行三维 (3D) 定量高分辨率成像。凭借其令人印象深刻的高分辨率（约 5 纳米），新型显微镜将能够以体积方式可视化厚的生物切片和其他物体，并将被耶鲁大学 11 个系的超过 17 个实验室使用。具体重点领域将包括神经元和突触、囊泡交通、细胞骨架和纳米材料的高分辨率成像，以及图像分析新方法的开发。这笔资助将在耶鲁大学科学界及其他领域产生广泛影响。它将帮助培训学生和博士后研究员使用强大的新成像技术，建立跨学科边界的新合作，将来自不同背景的科学家聚集在一起，与私营部门建立新的伙伴关系，开发分析和挖掘断层扫描数据集的方法，并为当地社区提供强有力的教育和推广计划（通过与当地高中和耶鲁大学“STARS”、SURF、Biostep 计划的链接）。 通过所有这些方式，该奖项将支持耶鲁大学和国家科学基金会的科学、教育和多样性目标。