MRI: Acquisition of High Capacity DNA Sequencing System

MRI：获取高容量 DNA 测序系统

• 批准号: 0821538
• 负责人: Michael Fromm
• 金额: $ 71.48万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821538&HistoricalAwards=false
• 关键词: MRI; Acquisition; Capacity; DNA; Sequencing

A grant has been awarded to the University of Nebraska (UNL) to purchase a next generation high throughput DNA sequencing system. This type of system has the capacity to obtain over 1 billion base pairs of DNA sequence per analysis. This tremendous amount of genetic information accelerates research in genomics projects in all the life sciences research programs at Nebraska. In particular, a new NSF-funded program on understanding how changes in chromatin modifications (epigenetics) affect gene expression will be able to obtain larger amounts of data more quickly using this machine. Additional uses will be to determine mRNA structures and expression in green algae, as a potential source of biofuels; to extend research on chromatin modifications into corn for the purpose of improving drought tolerance; and, on improving the understanding of how dietary molecules can improve human health through specific signaling pathways and changes in chromatin modifications. The range of new experimental opportunities provided by next generation high throughput sequencing systems is truly revolutionary. The high throughput sequencing capabilities will be integrated into student research projects and into UNL courses with an emphasis on facilitating the use of the technology by underrepresented minority and women graduate students. These fee-based services also will be broadly provided to the University of Nebraska campuses (UNL, UNMC, UNK and UNO), other universities, and local industry and organizations as well. The availability of such a critical genomics technology is also important for UNL?s recruiting of graduate and postdoctoral students as well as faculty.

内布拉斯加大学（UNL）获得了一笔赠款，用于购买下一代高通量DNA测序系统。这种类型的系统具有每次分析获得超过10亿个碱基对的DNA序列的能力。这一巨大数量的遗传信息加速了内布拉斯加州所有生命科学研究项目中基因组学项目的研究。特别是，一个新的NSF资助的项目，了解染色质修饰（表观遗传学）的变化如何影响基因表达，将能够使用这台机器更快地获得更大量的数据。其他用途将是确定作为生物燃料潜在来源的绿色藻类的mRNA结构和表达;将染色质修饰研究扩展到玉米，以提高耐旱性;以及提高对饮食分子如何通过特定信号传导途径和染色质修饰变化改善人类健康的理解。下一代高通量测序系统提供的一系列新的实验机会是真正革命性的。高通量测序能力将被整合到学生的研究项目和UNL课程中，重点是促进代表性不足的少数民族和女性研究生使用该技术。这些收费服务也将广泛提供给内布拉斯加大学校园（UNL，UNMC，UNK和UNO），其他大学，以及当地行业和组织。这样一个关键的基因组学技术的可用性也是很重要的UNL？招聘研究生和博士后以及教师。