A Protein Interaction Database For Rice Protein Kinases

谷物，包括水稻、玉米、小麦、大麦、高粱和谷子，是农业中最重要的一类植物，环境胁迫是其生产力的主要限制因素。提高它们对非生物和生物胁迫的耐受性是农业上的一个重要目标，在发现调节植物对这些胁迫反应的细胞信号机制方面已经取得了相当大的进展。该项目将通过生成275个大米蛋白激酶的蛋白质-蛋白质相互作用数据库来极大地促进这一进展，这些数据库代表了最近测序的水稻基因组中存在的每个蛋白激酶亚家族。蛋白质相互作用数据将使用酵母双杂交方法和一种利用将从转基因植物中纯化的亲和标记蛋白质复合体的质谱分析的方法来获得。此外，100个具有代表性的orotein激酶将通过插入突变或RNAi沉默来突变，并对它们的突变表型进行分析。这些综合信息将在PlantsP网站上提供，并将促进对细胞信号通路的理解。了解植物细胞用来调节其对环境胁迫的耐受性的机制，对于提高谷物的农业生产力至关重要。可食性：数据。项目数据将与基因注释一起存放在PlantsP网站(http://plantsp.sdsc.edu))。一旦完成和检查，全长的cDNA序列将被保存在Genbank和PlantsP中。蛋白质相互作用数据和基因敲除表型数据将在完成个别实验后每隔不超过六个月输入PlantsP数据库。材料。应Song实验室(美国佛罗里达大学宋文远)的要求，将提供水稻的cdna文库、BD-Kinase构建体和酵母双杂交诱饵阵列。他们将在一份出版物上做广告，这将在每年发生。这些文库的可用性也将在PlantsP网站和年度植物磷酸化会议上公布，该会议覆盖了大多数植物蛋白激酶社区。转基因水稻种子将从帕姆·罗纳德的实验室(加州大学戴维斯分校)或朱建康的实验室(亚利桑那大学)获得，直到有合适的水稻资源中心可用。

The cereals, including rice, maize, wheat, barley, sorghum, and the millets, are the most important group of plants in agriculture, and environmental stress is the major limitation to their productivity. Increasing their tolerance to abiotic and biotic stress is an important goal in agriculture and considerable progress has been made in discovering the mechanisms of cell signaling that regulate the response to these stresses in plants. This project will dramatically enhance this progress by generating a protein-protein interaction database for 275 rice protein kinases, that represent each of the protein kinase subfamilies present in the recently sequenced rice genome. The protein interaction data will be obtained using both the yeast two hybrid method and a method utilizing mass spectrometry analysis of affinity-tagged protein complexes that will be purified from transgenic plants. Additionally, 100 representative orotein kinases will be mutated by either insertional mutations or RNAi silencing, and their mutant phenotypes analyzed. This combined information will available at the PlantsP website and will advance the understanding of cell signaling pathways. The knowledge of the mechanisms that plant cells use to regulate their tolerance to environmental stress will be critical for improving the agricultural productivity of the cereals. Deliveables:Data. The project data will be deposited at the PlantsP website (http://plantsp.sdsc.edu) along with the gene annotations. Full-length cDNA sequences will be deposited in Genbank and PlantsP as soon as they are completed and checked. The protein interaction data, and knockout phenotype data will be entered into the PlantsP database at no longer than six month intervals after completing the individual experiments. Materials. The rice cDNA libraries, BD-kinase constructs, and yeast 2-hybrid bait arrays will be made available on request from the Song laboratory (Wen-Yuan Song, University of Florida). They will be advertised in a publication, which will occur in year. Availability of the libraries will also be advertised on the PlantsP website and at the annual plant phosphorylation meeting, which reaches most of the plant protein kinase community. Transgenic rice seeds will be made available from Pam Ronald's laboratory (UC Davis) or Jian-Kang Zhu's laboratory (Univeristy of Arizona) until a suitable rice resource center is available.