BRIGE: Understanding and Development of Bioseparation and Biosensing Schemes

BRIGE：生物分离和生物传感方案的理解和发展

• 批准号: 0823974
• 负责人: Susan Zhou
• 金额: $ 17.49万
• 依托单位: Worcester Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0823974&HistoricalAwards=false
• 关键词: BRIGE; Understanding; Development; Bioseparation; Biosensing

EEC-0823974ZhouThe proposed project will develop gold-coated magnetic nanoparticle (AuMNp)-based protein detection and separation and obtain fundamental understanding of magnetic field-induced microfluidics for efficient separation and rapid detection. Electrochemical Impedance Spectroscopy (EIS) and Surface Plasmon Resonance (SPR) will be used for the novel AuMNp-based protein detection. Finite element analysis will be performed to determine the effect of magnetic field, device geometry, fluid mixing, and reaction kinetics on protein transport and protein separation; and the effect of protein surface adsorption and binding, magnetic field and device geometry on the reaction rate of AuMNp -based protein binding under a magnetic field.The proposed work of using gold shell magnetic core nanoparticle for bioseparation and biosensing is unique in providing more robust and easy surface functionalization, high adsorption capacity, as well as amplified and high sensitive EIS and SPR signal. More importantly, the core/shell nanoparticle-based biosensing and bioseparation is also unique in that it has the potential of being used in magnetic field-induced microfluidics with integrated lab-on-a-chip bioseparation and biosensing capability. The biochip has the prospect of producing low-cost, rapid, hand-held devices for the practice of diagnostic medicine, the identification of biological weapons, the control of food safety and environmental pollutions, and basic life sciences research. The proposed work will result in fundamental understanding of magnetic field-induced AuMNp - based efficient protein separation and rapid protein detection in microchannel. This knowledge base will contribute significantly to the urgent need of the development of integrated hand-held microfluidic devices for proteomic and genomic analysis.The proposed research will produce a knowledge base for course development. The layered architecture of integrated research and mentoring for a continuum of students from middle school to graduate students will advance awareness of bionanotechnology to middle school students under the mentoring of undergraduate students (1-2 each summer). It will advance understanding and discovery through the second year program where graduate students, will be mentors. And, it will also provide opportunities to recruit and retain underrepresented students. The proposed research will have a society impact of addressing the compelling economic incentives for rapid, inexpensive and sensitive detection, for the diagnosis of disease markers, control of food safety and environmental pollutions or biohazards.

EEC-0823974周拟议的项目将开发基于镀金磁性纳米颗粒(AuMNp)的蛋白质检测和分离，并对磁场诱导微流控技术有基本的了解，以实现高效分离和快速检测。电化学阻抗谱(EIS)和表面等离子体共振(SPR)将被用于基于AuMNp的新型蛋白质检测。有限元分析将确定磁场、器件几何形状、流体混合和反应动力学对蛋白质传输和蛋白质分离的影响；以及蛋白质表面吸附和结合、磁场和器件几何形状对磁场下基于AuMNp的蛋白质结合反应速度的影响。更重要的是，基于核/壳纳米颗粒的生物传感和生物分离也是独一无二的，因为它具有集成的芯片实验室生物分离和生物传感能力，有可能用于磁场诱导的微流体。这种生物芯片有望生产出低成本、快速的手持设备，用于诊断医学、生物武器识别、食品安全和环境污染控制以及基础生命科学研究。这项工作将对磁场诱导的基于AuMNp的蛋白质高效分离和微通道中蛋白质的快速检测有基础的了解。该知识库将为蛋白质组和基因组分析手持式微流控一体化设备的开发提供重要的支持，并为课程开发提供知识库。从中学到研究生的综合研究和指导的分层架构将在本科生的指导下(每年夏天1-2次)提高中学生对生物科技的认识。它将通过研究生担任导师的二年级项目来促进理解和发现。此外，它还将为招收和留住代表性不足的学生提供机会。拟议的研究将产生社会影响，解决为快速、廉价和灵敏的检测、疾病标志物的诊断、食品安全和环境污染或生物危害的控制而采取的引人注目的经济激励措施。