Nanospiked Electrochemical Sensors in Acrylic Microfluidics for Clostridium difficile Toxin Detection

用于艰难梭菌毒素检测的丙烯酸微流体中的纳米尖峰电化学传感器

基本信息

  • 批准号:
    1805514
  • 负责人:
  • 金额:
    $ 34.99万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-07-01 至 2023-04-30
  • 项目状态:
    已结题

项目摘要

Clostridium difficile (C. difficile) is the most common infectious cause of diarrhea in hospitalized patients. Current commercially available tests cannot meet all the requirements of being rapid, low cost, sensitive, and selective. This proposed project will develop a novel platform to achieve the above goals. The success of the proposed research will make several broad scientific impacts. First, the proposed portable sensing platform will be extended for a broad range of applications. Second, it will enrich the fundamental knowledge of how nanostructured surfaces will affect the sensing performances. The acquired knowledge of nanostructured electrodes on the acrylic microfluidic platform can impact sensing research on other grand challenges, such as food safety and water systems. Research results will be disseminated to reach broader audiences by a) course development, b) incorporation into project-based learning for undergraduate and graduate students, and c) involvement of underrepresented groups, especially female students.This proposal aims to develop portable, easy-to-fabricate acrylic microfluidic devices based on novel electrochemical impedance spectroscopy (EIS) analysis of C. difficile enabled by nanospiked electrode surfaces. Specifically, we propose to combine engineering of nanospiked surfaces with a soft lithography technique to fabricate repeatedly controllable electrochemical electrode with low cost. We anticipate such electrodes will enhance the sensitivity thanks to the high area-to-volume ratio and small size of nanospikes on the surface. The nanospiked electrodes will be implemented on an acrylic-tape microfluidic platform following a simple fabrication process. The capability of the developed portable device for C. difficile sensing will be evaluated to guide the device deign. We anticipate the outcomes of the study will include 1) a thorough understanding of nanobiosensing performance, 2) a transformative method for engineering nanospiked electrochemical biosensors platforms, and 3) portable acrylic microfluidic devices with nanospiked electrodes for point-of-care biosensing of C. difficile.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
艰难梭菌(C. difficile)是住院患者腹泻最常见的感染性原因。目前市售的检测方法不能满足快速、低成本、灵敏和选择性的所有要求。这个项目将开发一个新颖的平台来实现上述目标。拟议研究的成功将产生若干广泛的科学影响。首先,提出的便携式传感平台将扩展到广泛的应用领域。其次,它将丰富纳米结构表面如何影响传感性能的基础知识。在丙烯酸微流控平台上获得的纳米结构电极的知识可以影响其他重大挑战的传感研究,如食品安全和水系统。研究成果将通过以下方式传播给更广泛的受众:a)课程开发;b)将研究成果纳入本科生和研究生的基于项目的学习;c)让代表性不足的群体,特别是女学生参与进来。该提案旨在开发便携式,易于制造的丙烯酸微流控装置,该装置基于纳米尖电极表面的新型电化学阻抗谱(EIS)分析艰难梭菌。具体而言,我们建议将纳米尖刺表面工程与软光刻技术相结合,以低成本制造可重复控制的电化学电极。我们预计,由于这种电极的高面积体积比和表面纳米尖峰的小尺寸,将提高灵敏度。纳米尖电极将在丙烯酸带微流控平台上通过简单的制造工艺实现。将评估所开发的便携式艰难梭菌检测设备的能力,以指导设备的设计。我们预计这项研究的结果将包括1)对纳米生物传感性能的透彻理解,2)工程纳米尖电化学生物传感器平台的变革方法,以及3)携带纳米尖电极的便携式丙烯酸微流控装置,用于艰难梭菌的即时生物传感。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Diagnostic methods and potential portable biosensors for coronavirus disease 2019
  • DOI:
    10.1016/j.bios.2020.112349
  • 发表时间:
    2020-10-01
  • 期刊:
  • 影响因子:
    12.6
  • 作者:
    Cui, Feiyun;Zhou, H. Susan
  • 通讯作者:
    Zhou, H. Susan
Development of Electrochemical 6-Well Plates and Its Stability as an Immunosensor
电化学 6 孔板的开发及其作为免疫传感器的稳定性
  • DOI:
    10.1149/1945-7111/ac519e
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    3.9
  • 作者:
    Cui, Feiyun;Zhou, Zhiru;Qu, Bin;Zhou, Qin;Zhou, H. Susan
  • 通讯作者:
    Zhou, H. Susan
Disposable Polyurethane Nanospiked Gold Electrode-Based Label-Free Electrochemical Immunosensor for Clostridium difficile
  • DOI:
    10.1021/acsanm.9b02001
  • 发表时间:
    2020-01-01
  • 期刊:
  • 影响因子:
    5.9
  • 作者:
    Cui, Feiyun;Zhou, Zhiru;Zhou, H. Susan
  • 通讯作者:
    Zhou, H. Susan
Reconfigurable Acrylic-tape Hybrid Microfluidics
  • DOI:
    10.1038/s41598-019-41208-y
  • 发表时间:
    2019-03-18
  • 期刊:
  • 影响因子:
    4.6
  • 作者:
    Ren, Yundong;Ray, Subhrodeep;Liu, Yuxiang
  • 通讯作者:
    Liu, Yuxiang
Batch fabrication of electrochemical sensors on a glycol-modified polyethylene terephthalate-based microfluidic device
  • DOI:
    10.1016/j.bios.2020.112521
  • 发表时间:
    2020-11-01
  • 期刊:
  • 影响因子:
    12.6
  • 作者:
    Cui, Feiyun;Jafarishad, Hamed;Zhou, H. Susan
  • 通讯作者:
    Zhou, H. Susan
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Susan Zhou其他文献

Healthcare Utilization and Chronic Disease Management for Non-Medicaid-Eligible Patients in a City-Wide Safety-Net Healthcare Access Program
  • DOI:
    10.1007/s11606-024-08983-5
  • 发表时间:
    2024-08-05
  • 期刊:
  • 影响因子:
    4.200
  • 作者:
    Kerry K. Meltzer;Kevin Chen;Christine Zhang;Susan Zhou;Theodore Long;Jonathan Jimenez
  • 通讯作者:
    Jonathan Jimenez
Incorporating K 12 Outreach Into An Reu Program For Females
将 K 12 外展纳入女性 Reu 计划
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T. Camesano;D. Dibiasio;K. Billiar;M. Rolle;Susan Zhou
  • 通讯作者:
    Susan Zhou
Intelligent and ligand-based surface-enhanced Raman spectroscopy biosensors for the instant screening of viral respiratory infections
  • DOI:
    10.1016/j.biosx.2024.100527
  • 发表时间:
    2024-10-01
  • 期刊:
  • 影响因子:
  • 作者:
    Bo Cao;Peng Lin;Yanling Wang;Weiwei Yang;Longxiang Ren;Zhongqiao Ge;Hongjing Sui;Yuan Gao;Mengyuan Liu;Guangcheng Bei;Susan Zhou;Qin Zhou;Feiyun Cui
  • 通讯作者:
    Feiyun Cui
Substance use and its relationship to depression, anxiety, and isolation among youth living with HIV
艾滋病毒青少年的药物使用及其与抑郁、焦虑和孤立的关系
  • DOI:
    10.1207/s15327558ijbm0604_1
  • 发表时间:
    1999
  • 期刊:
  • 影响因子:
    2.7
  • 作者:
    M. Rotheram;D. Murphy;D. Swendeman;B. Chao;B. Chabon;Susan Zhou;J. Birnbaum;P. O'hara
  • 通讯作者:
    P. O'hara
V2CTx MXene-powered handheld SERS biosensor for the viral antigen test
  • DOI:
    10.1007/s12598-025-03408-6
  • 发表时间:
    2025-06-30
  • 期刊:
  • 影响因子:
    11.000
  • 作者:
    Meng-Nan Li;Bo Cao;Yan-Ling Wang;Hang Zhou;Hai-Xu Yao;Yuan Gao;Xi-Wei Zhuang;Jian-Lei Liu;Chi-Jia Zeng;Susan Zhou;Da-Ling Zhu;Jian Ma;Fei-Yun Cui
  • 通讯作者:
    Fei-Yun Cui

Susan Zhou的其他文献

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{{ truncateString('Susan Zhou', 18)}}的其他基金

International: Undergraduate Research Opportunities on Water Treatment
国际:水处理本科研究机会
  • 批准号:
    1130202
  • 财政年份:
    2011
  • 资助金额:
    $ 34.99万
  • 项目类别:
    Standard Grant
Engineering Rapid and Ultrasensitive Microsystem with Hybrid Nanomaterials
使用混合纳米材料设计快速、超灵敏的微系统
  • 批准号:
    1030289
  • 财政年份:
    2010
  • 资助金额:
    $ 34.99万
  • 项目类别:
    Standard Grant
BRIGE: Understanding and Development of Bioseparation and Biosensing Schemes
BRIGE:生物分离和生物传感方案的理解和发展
  • 批准号:
    0823974
  • 财政年份:
    2008
  • 资助金额:
    $ 34.99万
  • 项目类别:
    Standard Grant
International Research and Education: Planning Visits to Shanghai Jiao Tong University (SJTU), PRC
国际研究与教育:计划访问中国上海交通大学 (SJTU)
  • 批准号:
    0805856
  • 财政年份:
    2008
  • 资助金额:
    $ 34.99万
  • 项目类别:
    Standard Grant

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Low-cost electrochemical sensors derived from food waste for sensitive in-situ detection of water contaminants
来自食物垃圾的低成本电化学传感器,用于水污染物的灵敏原位检测
  • 批准号:
    2890356
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    2023
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    2023
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Development of Electrochemical Sensors for Chronic Disease Biomarkers
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  • 批准号:
    573902-2022
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Nanomaterials-based Electrochemical Keytone Sensors
基于纳米材料的电化学基调传感器
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SCC-IRG Track 1: Community Based Approach to Address Contaminants in Drinking Water using Smart Cloud-Connected Electrochemical Sensors
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