BRIGE: Protein-surfactant nanostructures for enhanced electrophoretic separations

BRIGE：用于增强电泳分离的蛋白质表面活性剂纳米结构

• 批准号: 0824347
• 负责人: Lilo Pozzo
• 金额: $ 17.5万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0824347&HistoricalAwards=false
• 关键词: BRIGE; Protein; surfactant; nanostructures; enhanced

0824347PozzoThe main goal of electrophoretic separation is to use electric fields to separate proteins out of complex biological mixtures (e.g. serum, saliva or cell lysates). The proposed work uses the principles of nanoscience to rationally improve reproducibility, resolution, and sensitivity.. It focuses on the structure of the protein-surfactant complex and employs a multi-scale experimental approach based on the fundamentals of colloid and polymer science to decipher the intricate relationship between the nano-structure of protein-surfactant complexes, the mechanism of electrophoretic transport (e.g. reptation) and the effectiveness of the macroscopic separation.Light, x-ray and neutron scattering experiments will systematically evaluate the structure of protein complexes that are made from "non traditional" anionic surfactants. These experiments will probe nanometer scale structural transitions occurring in the protein-surfactant complex. The changes are triggered by specific alterations in the hydrophobic moiety of the surfactant (e.g. linear and branched alkyl chains) or through the use of mixed surfactant and co-surfactant formulations. In addition, the structure and conformation of the proteins will be monitored in-situ, using on-line neutron scattering experiments carried out in polymeric gels and with the application of electric fields. This should lead to the development of an accurate physical understanding of the true structural parameters that govern electrophoretic transport. The long term goal is to extend these studies into other areas, such as the use of nano-structured sieving matrices, so that we can develop a complete understanding of the separation. Educationally the results of this work will be incorporated into existing courses to demonstrate the use of surfactants in high-technology applications. It will immediately impact the research experience of graduate and undergraduate students from under-represented groups. This will help to increase their participation in STEM graduate programs. Finally, the proposal will allow the PI to continue serving as a liaison between the NIST Center for Neutron Research and faculty and students from under-represented groups. This will increase awareness of these and other valuable resources among groups that have historically been unable to take advantage of their availability.

0824347电泳分离的主要目标是使用电场将蛋白质与复杂的生物混合物（例如血清，唾液或细胞裂解物）分开。 拟议的工作使用纳米科学的原理来合理地改善可重复性，解决和敏感性。它的重点是蛋白质 - 依赖性剂络合物的结构，并采用基于胶体和聚合物科学基础的多尺度实验方法来实现蛋白质构造和蛋白质 - 依然复杂的机制，以及蛋白质 - 依从性复杂的机制，并具有质量的机制，并具有蛋白质的机制，并具有质量依赖性的机制。宏观分离的有效性。光明，X射线和中子散射实验将系统地评估由“非传统”阴离子表面活性剂制成的蛋白质复合物的结构。这些实验将探测纳米尺度的结构过渡，发生在蛋白质表面活性剂复合物中。这些变化是由表面活性剂（例如线性和分支烷基链）或使用混合表面活性剂和共同表面活性剂配方的特异性改变引起的。此外，将使用在聚合物凝胶中进行的在线中子散射实验并使用电场的应用，将对蛋白质的结构和构象进行监测。这应该导致对控制电泳运输的真实结构参数的准确理解。 长期目标是将这些研究扩展到其他领域，例如使用纳米结构化的筛分矩阵，以便我们可以完全了解分离。在教育上，这项工作的结果将被纳入现有课程中，以证明在高科技应用中使用表面活性剂。 这将立即影响来自代表性不足的小组的研究生和本科生的研究经验。这将有助于增加他们参与STEM研究生课程。最后，该提案将允许PI继续作为NIST中子研究中心与教职员工与来自代表性不足的团体的学生之间的联络。这将提高人们对这些和其他有价值的资源的认识，而这些群体历来无法利用它们的可用性。