CAREER: Dissecting the Regulation of Ribosome Assembly by the Essential GTPase Bms1

职业：剖析必需的 GTPase Bms1 对核糖体组装的调节

• 批准号: 0845156
• 负责人: Katrin Karbstein
• 金额: $ 69.4万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0845156&HistoricalAwards=false
• 关键词: CAREER; Dissecting; Regulation; Ribosome; Assembly

Intellectual MeritProteins are the functional entities in all cells. They are synthesized on large molecular machines, called ribosomes. Ribosomes themselves consist of 78 proteins and 4 RNAs, incorporated into large particles. Cellular function depends on ribosome accurately translating the information contained in the genetic code into proteins. This, in turn, requires the correct assembly of ribosomes. One way in which correct ribosome synthesis is achieved is by ordering steps, so that a later step depends on the prior steps being accurate. This project investigates how a regulatory protein, called Bms1, uses energy to regulate and order essential steps in ribosome assembly. These questions will be addressed in a combination of biochemical, kinetic and genetic experiments. Insights garnered from this work will also have implications for other cellular processes that involve the dynamic assembly of RNA-protein complexes, including pre-mRNA splicing and RNAi. Broader ImpactFaculty members at large universities have two important, sometimes competing, missions in educating young scientists. Class-room teaching is meant to convey already established knowledge to undergraduate and graduate students, while young scientists are raised and educated in the lab. Many graduate students at the University of Michigan have a strong interest in teaching careers, which makes it important to connect these two missions by raising excellent scientists that are also well-trained as teachers. The educational part of the project will develop a lecture series called Scientific Discovery. This lecture series will introduce undergraduate students to cutting edge research, both to help them make the connection between lab and textbook, but also to spark their interest in science. This lecture series is specifically designed to involve senior graduate students and postdocs in teaching at the undergraduate level (not just via Graduate Student Instructor positions). Furthermore, the new Techniques in Biochemistry Lab Course will introduce the web-based enzymology teaching tool webZyme. This novel instrument will give students the opportunity to "perform experiments" that they would normally not be able to do, and introduce the Graduate Student Instructor to this exciting new tool. It is expected that a virtual enzymology lab might be especially valuable in the small undergraduate institutions that many of our students have identified as their "ideal" future employers, thus enhancing their job prospects as well as their effectiveness as future teachers.

蛋白质是所有细胞中的功能实体。它们在称为核糖体的大分子机器上合成。核糖体本身由78种蛋白质和4种RNA组成，它们被整合成大颗粒。细胞功能依赖于核糖体将遗传密码中包含的信息准确地翻译成蛋白质。这反过来又需要核糖体的正确组装。实现正确的核糖体合成的一种方法是对步骤进行排序，使得后面的步骤取决于前面的步骤是否准确。该项目研究了一种名为Bms 1的调节蛋白如何利用能量来调节和排序核糖体组装中的重要步骤。这些问题将在生物化学，动力学和遗传实验的组合中得到解决。从这项工作中获得的见解也将对其他涉及RNA-蛋白质复合物动态组装的细胞过程产生影响，包括前体mRNA剪接和RNAi。 更广泛的影响大型大学的教师在教育年轻科学家方面有两个重要的，有时是相互竞争的任务。课堂教学的目的是向本科生和研究生传授已经建立的知识，而年轻的科学家则是在实验室中培养和教育的。密歇根大学的许多研究生对教学职业有着浓厚的兴趣，这使得通过培养优秀的科学家来将这两项任务联系起来变得非常重要。该项目的教育部分将开发一个名为科学发现的系列讲座。本系列讲座将向本科生介绍前沿研究，既帮助他们建立实验室和教科书之间的联系，又激发他们对科学的兴趣。这个系列讲座是专门设计的，涉及高年级研究生和博士后在本科教学水平（而不仅仅是通过研究生导师职位）。此外，新的生物化学实验技术课程将介绍基于网络的酶学教学工具webZyme。这种新颖的仪器将使学生有机会“执行实验”，他们通常不能做，并介绍研究生导师这个令人兴奋的新工具。预计虚拟酶学实验室可能是特别有价值的小型本科院校，我们的许多学生已经确定为他们的“理想”未来的雇主，从而提高他们的就业前景以及他们的效率作为未来的教师。