Plasmonic Photothermal Surgery on Fragile Living Cells

对脆弱活细胞进行等离子光热手术

• 批准号: 0853500
• 负责人: Pei-Yu Chiou
• 金额: $ 50.5万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0853500&HistoricalAwards=false
• 关键词: Plasmonic; Photothermal; Surgery; Fragile; Living

0853500ChiouThis proposal aims to develop a live, single-cell somatic and stem cell surgery toolenabling high efficiency intracellular manipulations, such as the delivery of small molecules, macromolecular structures, and organelles like mitochondria. The long term goal is to enhance capabilities in stem cell biology for the repair of damaged or worn out tissues due to congenital dysfunction, aging, or the ravages of disease, such as HIV/AIDS, cancer, diabetes, and atherosclerosis. Developing this ability is one of the main challenges in the stem cell research community. To approach the problems associated with organelle/small molecule delivery and intracellular manipulations of live, fragile federally-approved embryonic stem cells, they propose to develop a novel cell surgery device. This device integrates conventional microcapillary techniques with plasmonic photothermal technologies to precisely control nanoscale cell membrane damage near the tip of a microcapillary pipette. This fine control is realized byutilizing the unique photothermal effect of metal nanoparticles or thin films coated on the tip of microcapillary pipettes, which can therefore utilize a larger bore pipette diameter for efficient and non-shearing transfer of larger items such as mitochondria into somatic and stem cells.

0853500 Chiou该提案旨在开发一种活的单细胞体细胞和干细胞手术工具，能够实现高效的细胞内操作，例如递送小分子、大分子结构和线粒体等细胞器。长期目标是增强干细胞生物学的能力，用于修复因先天性功能障碍、衰老或疾病（如HIV/AIDS、癌症、糖尿病和动脉粥样硬化）造成的受损或磨损组织。开发这种能力是干细胞研究界的主要挑战之一。为了解决与细胞器/小分子递送和活的、脆弱的联邦批准的胚胎干细胞的细胞内操作相关的问题，他们提议开发一种新型的细胞手术装置。该装置将传统的微毛细管技术与等离子体光热技术相结合，以精确控制微毛细管移液器尖端附近的纳米级细胞膜损伤。这种精细控制是通过利用涂覆在微毛细管移液器尖端上的金属纳米颗粒或薄膜的独特光热效应来实现的，因此可以利用更大口径的移液器直径来有效和非剪切地将更大的物品（例如线粒体）转移到体细胞和干细胞中。