New Molecularly Imprinted Polymers with Significantly Enhanced Properties for Chemical and Biological Analyses and Separations

新型分子印迹聚合物具有显着增强的化学和生物分析与分离性能

• 批准号: 0854105
• 负责人: David Spivak
• 金额: $ 28.64万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0854105&HistoricalAwards=false
• 关键词: New; Molecularly; Imprinted; Polymers; Significantly

0854105SpivakOne monomer molecularly imprinted polymers (referred to as 'OMNiMIPs'), synthesized from novel crosslinking monomers developed in the PI's group, exhibit fundamental differences that give rise to new and enhanced properties versus traditionally formed molecularly imprinted polymers (MIPs). First, the use of one monomer ultimately affords a higher loading capacity per gram of polymer, which can improve applications such as solid phase extraction and analytical separations. The increased rebinding capacity of OMNiMIPs could lead to applications beyond current limits of MIPs, such as high capacity removal of target compounds in solid phase extraction, environmental clean-up, or protein-purification strategies. We are currently investigating the underlying principles that are responsible for the high capacity and selectivity of OMNiMIPs. The higher capacity also affords higher performance for imprinting several different target compounds simultaneously, facilitating assays and separations of multiple analytes. This could be important for assays on mixtures of different compounds, such as the analysis of pharmaceutical formulations or solid phase extraction of an array of different molecules. In addition, novel chiral OMNiMIPs appear capable of imprinting racemic mixtures, providing an easier route to chiral stationary phases. This would eliminate the need for enantiopure templates, normally required for traditional MIPs. Last, OMNiMIPs will be hybridized with conjugated polymers to provide a material that can generate a fluorescence signal upon binding the target molecule, which is useful for sensors and other detection strategies. An important theme for the research described in this proposal is to utilize molecular control of the monomer starting materials toward optimizing macroscopic properties (e.g. loading capacity and selectivity) of OMNiMIPs. The heart of this proposal is the design, synthesis and evaluation of new crosslinkers for the OMNiMIP process that will maximize the performance of these materials for the applications described above. Using the molecular structure of successful crosslinkers as lead compounds, analogs with rational changes will be evaluated for improvements in structure-property relationships. This proposal describes the fundamental development of new MIP materials that both improve performance and are capable of unique applications that have not been available from this technology in the past. It is important to determine the optimal crosslinker structures as quickly as possible in order to provide researchers worldwide with the best materials for their applications. For society, the promise of molecular imprinting is the detection of medical and environmental toxins, detection and neutralization of national security target compounds, tailored catalysts, and separation media that will serve the health and advancement of mankind in numerous ways. These broader impacts of molecular imprinting will be enhanced in several ways by the development of OMNiMIPs for novel applications such as those described above. While the development of these materials should be seen as a major practical outcome of this research, these studies will also contribute to the fundamental understanding of the underlying principles of the OMNiMIP effect and how these differ from traditional molecular imprinting approaches. Furthermore, the development of OMNiMIPs has stimulated numerous collaborations with researchers, both nationally and internationally, resulting in many interesting studies toward the increased performance of these materials for current and new applications. OMNiMIP research has also provided opportunities for undergraduates and a high school student to participate in cutting edge research and present their results at conferences. The real credit for the success and fun of OMNiMIP research belongs to the diverse group of graduate students and post-doctoral fellows who have engaged themselves in the day-to-day problem solving that has made this research flourish. Our group is talented and draws evenly from underrepresented groups that are mentored well here at LSU. Their training in molecular imprinting and OMNiMIPs continues to present opportunities that interface important chemical disciplines naturally, providing an interdisciplinary education that will enhance their competitiveness in the current and future marketplace. The people and the research in this overall program will reach the broader community in Louisiana, especially younger generations, through public lectures and presentations with the goal to improve their awareness and interest of basic science and technology.

0854105 SpivakOne单体分子印迹聚合物（称为“OMNiMIPs”），由PI组开发的新型交联单体合成，与传统形成的分子印迹聚合物（MIPs）相比，表现出根本性的差异，从而产生新的和增强的性能。首先，使用一种单体最终提供了每克聚合物更高的负载容量，这可以改善诸如固相萃取和分析分离的应用。OMNiMIPs的增加的再结合能力可能导致超越当前MIPs限制的应用，例如在固相萃取、环境净化或蛋白质纯化策略中高容量去除目标化合物。我们目前正在调查的基本原则，负责高容量和选择性的OMNiMIP。更高的容量也提供了更高的性能，同时印迹几种不同的目标化合物，促进分析和分离多种分析物。这对于不同化合物的混合物的分析可能是重要的，例如药物制剂的分析或不同分子阵列的固相萃取。此外，新的手性OMNiMIPs似乎能够印迹外消旋混合物，提供了一个更容易的途径，手性固定相。这将消除对传统MIP通常所需的对映体纯模板的需要。最后，OMNiMIPs将与共轭聚合物杂交，以提供一种可以在结合目标分子时产生荧光信号的材料，这对于传感器和其他检测策略是有用的。 本提案中描述的研究的一个重要主题是利用单体起始材料的分子控制来优化OMNiMIPs的宏观性质（例如负载能力和选择性）。该提案的核心是设计、合成和评估用于OMNiMIP工艺的新交联剂，其将最大限度地提高这些材料用于上述应用的性能。使用成功的交联剂的分子结构作为先导化合物，将评估具有合理变化的类似物的结构-性质关系的改善。该提案描述了新MIP材料的基本发展，这些材料既提高了性能，又能够实现过去该技术无法实现的独特应用。尽快确定最佳交联剂结构非常重要，以便为全球研究人员提供最佳材料。 对于社会来说，分子印迹的前景是检测医疗和环境毒素，检测和中和国家安全目标化合物，定制催化剂和分离介质，这些将以多种方式为人类的健康和进步服务。这些更广泛的影响，分子印迹将提高在几个方面的发展OMNiMIP的新的应用，如上述。虽然这些材料的开发应该被视为这项研究的主要实际成果，但这些研究也将有助于对OMNiMIP效应的基本原理以及这些与传统分子印迹方法的不同之处的基本理解。此外，OMNiMIPs的开发激发了国内外研究人员的众多合作，导致许多有趣的研究，以提高这些材料在当前和新应用中的性能。OMNiMIP研究还为本科生和高中生提供了参与前沿研究并在会议上展示成果的机会。OMNiMIP研究的成功和乐趣的真实的功劳属于研究生和博士后研究员的多样化群体，他们从事日常问题的解决，使这项研究蓬勃发展。我们的团队是有才华的，并从代表性不足的群体，在路易斯安那州立大学得到很好的指导。他们在分子印迹和OMNiMIP的培训继续提供机会，自然地接口重要的化学学科，提供跨学科的教育，将提高他们在当前和未来市场的竞争力。在这个整体计划的人和研究将达到路易斯安那州更广泛的社区，特别是年轻一代，通过公开讲座和演示，以提高他们的认识和基础科学和技术的兴趣的目标。