Phosphoproteins in the primed defense response of plants

植物启动防御反应中的磷蛋白

• 批准号: 192597169
• 负责人: Professor Dr. Uwe Conrath
• 金额: --
• 依托单位: Max-Planck-Institut für Pflanzenzüchtungsforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/192597169?language=en
• 关键词: Phosphoproteins; primed; defense; response; plants

Plants can be primed for more rapid and robust activation of defense which often comes with induced immunity. In Arabidopsis thaliana defense priming requires heat shock transcription coactivator HsfB1, histone modifications on defense gene promoters, and accumulation of mitogen-activated protein kinases MPK3 and MPK6 (at both the mRNA transcript and protein levels). However, MPK3/6 are maintained in an inactive state in primed cells and require pathogen challenge for activation. Upon pathogen challenge more MPK3/6 enzymes are activated in primed than in nonprimed cells. The activation of more MPKs in primed plants correlates with enhanced defense gene expression and induction of immunity. Thus, MPK3/6 are important regulators of priming in Arabidopsis. However, other phosphoproteins important for priming remained elusive. We developed a powerful, tandem metal oxide affinity chromatography approach for the extraction, identification, and quantification by LC/MS of low abundant, transiently phosphorylated proteins with a likely role in priming. In the present proposal we aim to characterize, and functionally analyze selected novel phosphoprotein candidates (e.g. PEARLI4 and TFIIS) that are important for defense priming in Arabidopsis.

植物可以为更快速、更强大的防御激活做好准备，这通常伴随着诱导免疫。在拟南芥中，防御启动需要热休克转录辅活化子HsfB1、防御基因启动子上的组蛋白修饰以及丝裂原活化蛋白激酶MPK3和MPK6的积累(在mRNA转录和蛋白水平上)。然而，MPK3/6在启动的细胞中保持非活性状态，需要病原体攻击才能激活。在病原体攻击时，预激细胞比非预激细胞激活更多的MPK3/6酶。在被启动的植物中，更多的MPK被激活，这与增强防御基因的表达和诱导免疫有关。因此，MPK3/6在拟南芥中是启动的重要调节因子。然而，其他对启动至关重要的磷蛋白仍然难以捉摸。我们开发了一种功能强大的串联金属氧化物亲和层析方法，用于提取、鉴定和LC/MS定量低丰度的瞬时磷酸化蛋白，这些蛋白可能在启动过程中发挥作用。在目前的建议中，我们的目标是鉴定和功能分析在拟南芥中对防御启动起重要作用的新的候选磷蛋白(例如PEARLI4和TFIIS)。