LIGHT-TRANSDUCTION IN MELANOPSIN-EXPRESSING PHOTORECEPTORS OF AMPHIOXUS Mechanistic analysis and evolutionary implications

文昌鱼表达黑色素的光感受器的光传导机制分析和进化意义

• 批准号: 0918930
• 负责人: Maria del Pilar Gomez
• 金额: $ 53.51万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0918930&HistoricalAwards=false
• 关键词: LIGHT; TRANSDUCTION; MELANOPSIN; EXPRESSING; PHOTORECEPTORS

"This award is funded under the American Recovery and Reinvestment Act of 2009(Public Law 111-5)."Animal vision was traditionally thought to be comprised of two distinct lineages of sensory cells that evolved separately in vertebrates vs. invertebrates. These photoreceptors differ fundamentally in the light sensor structure and the biochemistry to convert captured photons into electrical signals. Such view was challenged by the discovery of additional light-detection schemes and by clues pointing to a likely common origin for all photoreceptors. One pivotal finding was the identification of novel light-sensitive mammalian cells, which regulate the biological clock via a photon-capturing molecule, melanopsin, akin to those of invertebrates. However, the enormous divergence between these groups of animals makes comparative analysis arduous, exacerbated by the difficulties to characterize melanopsin-signaling in mammalian cells, owing to their scarcity. This impasse could be alleviated by examining some precursor of modern vertebrates, to bridge this evolutionary chasm. Amphioxus offers unique advantages, because genomic research established that it is the most basal living chordate and has remained close to its ancestral condition. It thus provides a favorable window to examine biological mechanisms as they may have existed when the vertebrate branch separated. Melanopsin has been detected in some identifiable amphioxus cells, but no functional study had been conducted; the investigators will capitalize on their initial results, demonstrating the feasibility to isolate these cells and measure light-induced electrical responses. The project will recruit and train graduate students at the Marine Biological Laboratory in Woods Hole. The Marine Biological Laboratory provides an outstanding environment for this work. A multi-pronged approach will characterize the photoresponse mechanisms, identify intermediate steps of stimulus-response coupling, and initiate their molecular characterization. The benefits will be two-fold: help clarify the evolution of light-sensing, and elucidate melanopsin signaling mechanisms, which remain largely elusive. The multi-disciplinary nature of the project provides a fertile ground to train young investigators, exposing them to issues ranging from evolutionary biology to sensory physiology and cellular biophysics; the technical arsenal will be correspondingly diverse, encompassing electrical and optical recording, molecular and immunological identification, and bio-informatics tools.

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。“动物视觉传统上被认为是由两种不同的感觉细胞谱系组成，它们分别在脊椎动物和无脊椎动物中进化。 这些光感受器在光传感器结构和将捕获的光子转换为电信号的生物化学方面有根本的不同。 这一观点受到了新发现的光探测机制的挑战，并有线索指出，所有光感受器可能都有共同的起源。 一个关键的发现是鉴定出了新的光敏哺乳动物细胞，这些细胞通过一种光子捕获分子黑视蛋白来调节生物钟，类似于无脊椎动物的细胞。 然而，这些动物群体之间的巨大差异使得比较分析变得困难，由于它们的稀缺性，难以描述哺乳动物细胞中的黑视素信号传导。 这一僵局可以通过研究现代脊椎动物的某些前身来缓解，以弥合这一进化鸿沟。 文昌鱼提供了独特的优势，因为基因组研究表明，它是最基本的生活脊索动物，并保持接近其祖先的条件。 因此，它提供了一个有利的窗口，以检查生物学机制，因为它们可能已经存在时，脊椎动物分支分离。 在一些可识别的文昌鱼细胞中检测到黑视素，但尚未进行功能研究;研究人员将利用他们的初步结果，证明分离这些细胞并测量光诱导电反应的可行性。 该项目将在伍兹霍尔的海洋生物实验室招募和培训研究生。 海洋生物实验室为这项工作提供了一个出色的环境。 多管齐下的方法将表征光响应机制，识别刺激-响应耦合的中间步骤，并启动其分子表征。 它的好处是双重的：有助于阐明感光的进化，并阐明黑视素信号传导机制，这在很大程度上仍然是难以捉摸的。 该项目的多学科性质为培训年轻的研究人员提供了肥沃的土壤，使他们接触到从进化生物学到感觉生理学和细胞生物物理学的问题;技术武器库将相应地多样化，包括电子和光学记录，分子和免疫学鉴定以及生物信息学工具。