RUI: Transcriptional Regulation of INO1 in Yeast

RUI：酵母中 INO1 的转录调控

• 批准号: 0919218
• 负责人: Chang-Hui Shen
• 金额: $ 27.65万
• 依托单位: CUNY College of Staten Island
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919218&HistoricalAwards=false
• 关键词: RUI; Transcriptional; Regulation; INO1; Yeast

Several phospholipids, particularly inositol-containing phospholipids, have been implicated in many complex signaling processes, which play a role in controlling cell growth and proliferation in higher eukaryotes. The mechanisms by which eukaryotic cells control the synthesis of membrane lipids in coordination with ongoing membrane biogenesis are not yet fully understood. The first step in understanding these complex phenomena is to examine the regulation of phospholipid gene expression. In order to understand this regulation, the precise details of the chromatin structure and relevant factors involved in the transcriptional activation process must be unraveled. Yeast cells synthesize a typical eukaryotic mixture of phospholipids, using pathways which are similar to those in higher eukaryotes. The goal of this research is to use the genetic and biochemical tools available for Saccharomyces cerevisiae to investigate the mechanism of INO1 expression. INO1 encodes inositol-1-phosphate synthase, which catalyzes the rate-limiting step in the synthesis of inositol. INO1 expression is required for the de novo synthesis of phosphatidylinositol, which has been implicated in complex signaling processes, such as mRNA export, vesicular trafficking, and cell growth and proliferation. This project addresses questions pertaining to the possible roles of the transcriptional activator, Ino2p, during INO1 activation. These experiments will examine how chromatin remodelers and histone modifying enzymes are recruited to the INO1 promoter, and identify the functional residue(s) in Ino2p, which is/are responsible for recruiting remodelers and/or histone modifying enzymes. Therefore, this research will provide new insight into the mechanism of gene regulation and the signal transduction pathway of phospholipids. Broader impacts: This project will provide an excellent opportunity for undergraduate students to be actively engaged in cutting-edge research. Students will be trained to perform experiments, leading to new discoveries related to gene expression. During the course of study, undergraduate researchers will gain experience in biochemical and genetic techniques, creative problem solving, and working together as a team. The ability to integrate the knowledge they have acquired in the classroom with hands-on, exciting scientific research will provide an invaluable experience for undergraduate students. Furthermore, laboratory research experience can provide students with the opportunity to develop and acquire rigorous scientific methodologies. Thus, these students will be equipped with both fundamental and comprehensive knowledge that they may then apply in solving other biological questions.

几种磷脂，特别是含有肌醇的磷脂，已经涉及许多复杂的信号传导过程，其在控制高等真核生物的细胞生长和增殖中起作用。 真核细胞控制膜脂合成与膜生物合成的机制尚未完全清楚。 理解这些复杂现象的第一步是检查磷脂基因表达的调节。 为了理解这种调节，必须解开染色质结构和转录激活过程中涉及的相关因子的精确细节。 酵母细胞合成磷脂的典型真核混合物，使用类似于高等真核生物中的途径。本研究的目的是利用酿酒酵母的遗传和生化工具来研究INO 1表达的机制。 INO 1编码肌醇-1-磷酸合酶，其催化肌醇合成中的限速步骤。 INO 1的表达是磷脂酰肌醇从头合成所必需的，磷脂酰肌醇参与了复杂的信号传导过程，如mRNA输出、囊泡运输以及细胞生长和增殖。 该项目解决了有关转录激活因子Ino 2 p在INO 1激活过程中可能发挥的作用的问题。 这些实验将检查染色质重塑和组蛋白修饰酶如何被募集到INO 1启动子，并鉴定Ino 2 p中负责募集重塑和/或组蛋白修饰酶的功能残基。 因此，这项研究将为磷脂的基因调控机制和信号传导途径提供新的见解。 更广泛的影响：该项目将为本科生提供一个积极参与前沿研究的绝佳机会。 学生将接受训练，进行实验，导致有关基因表达的新发现。在学习过程中，本科研究人员将获得生物化学和遗传技术，创造性解决问题，并作为一个团队一起工作的经验。 将他们在课堂上获得的知识与动手，令人兴奋的科学研究相结合的能力将为本科生提供宝贵的经验。此外，实验室研究经验可以为学生提供发展和获得严格的科学方法的机会。 因此，这些学生将具备基础和综合知识，然后他们可以应用于解决其他生物学问题。