Role of the Arabidopsis thaliana TPS11 gene and trehalose metabolism in defense against green peach aphid

拟南芥TPS11基因和海藻糖代谢在防御桃蚜中的作用

• 批准号: 0919192
• 负责人: Jyoti Shah
• 金额: $ 64万
• 依托单位: University of North Texas
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919192&HistoricalAwards=false
• 关键词: Role; Arabidopsis; thaliana; TPS11; gene

Aphids are a large group of phloem-feeding insects that cause extensive damage to plants and vector important viruses. However, our understanding of plant defense against aphids is limited. The interaction between Arabidopsis thaliana and Myzus persicae, commonly known as green peach aphid (GPA), has uncovered a role for the Arabidopsis TPS11 gene, which exhibits homology to trehalose metabolism genes, in plant defense against aphids. The goal of this project is to characterize the involvement of TPS11 in trehalose metabolism, and the role of trehalose metabolism in plant defense against aphids. A combination of biochemical, molecular, genetic and genomic tools combined with insect bioassays and behavioral studies will be utilized to accomplish these goals. Since trehalose and its metabolites are present at low levels in plants, it is anticipated that this study will uncover a regulatory function of trehalose metabolism in plants. The proposed activity will enhance understanding of plant defense against GPA, which is a pest of 50 plant families and vectors several important viruses. In addition, it will fill important gaps in our knowledge of trehalose metabolism and its involvement in plant stress response. Broader Impacts: This project will benefit a graduate student and a post-doctoral fellow who will receive training in biochemistry, molecular biology and genetics. In addition, through the PI's instructional activities this project will bring current knowledge of plant signaling, carbohydrate metabolism and stress response into the classroom. This project will also provide an opportunity to train undergraduates and high school students and broaden the participation of underrepresented groups in research. Results from this project will also have potential benefits to society at large, because they will identify steps in plant metabolism that mediate defense against insects. In the future, manipulation of these steps may augment aphid resistance and enhance quality and productivity of plants.

蚜虫是一大群取食韧皮部的昆虫，对植物造成广泛的损害并传播重要的病毒。 然而，我们对植物防御蚜虫的理解是有限的。 拟南芥和桃蚜（Myzus persicae）（通常称为绿色桃蚜（GPA））之间的相互作用揭示了拟南芥TPS11基因在植物防御蚜虫中的作用，该基因与海藻糖代谢基因具有同源性。 本项目的目的是表征TPS11在海藻糖代谢中的参与，以及海藻糖代谢在植物防御蚜虫中的作用。 生物化学，分子，遗传和基因组工具结合昆虫生物测定和行为研究的组合将被用来实现这些目标。由于海藻糖及其代谢产物在植物中的含量较低，预计这项研究将揭示植物海藻糖代谢的调节功能。 拟议的活动将加强对植物防御GPA的理解，GPA是50个植物科的害虫，并携带几种重要病毒。 此外，它将填补重要的空白，我们的知识海藻糖代谢和参与植物胁迫反应。 更广泛的影响：该项目将使一名研究生和一名博士后受益，他们将接受生物化学、分子生物学和遗传学方面的培训。 此外，通过PI的教学活动，该项目将把植物信号，碳水化合物代谢和压力反应的当前知识带入课堂。 该项目还将提供一个机会，培训本科生和高中生，扩大代表性不足的群体参与研究。 该项目的结果也将对整个社会产生潜在的好处，因为它们将确定植物代谢中介导防御昆虫的步骤。 在未来，这些步骤的操作可能会增强蚜虫的抗性，提高植物的质量和生产力。