Collaborative Research: Metabolomic Profiling and Functions of Oxidized Membrane Lipids in Plant Stress Responses

合作研究：氧化膜脂质在植物胁迫反应中的代谢组学分析和功能

• 批准号: 0920600
• 负责人: Jyoti Shah
• 金额: $ 40.05万
• 依托单位: University of North Texas
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920600&HistoricalAwards=false
• 关键词: Collaborative; Research; Metabolomic; Profiling; Functions

Metabolomic profiling and functions of oxidized membrane lipids in plant stress responses Ruth Welti and Gary L. Gadbury, Kansas State University Jyoti Shah, University of North Texas Xuemin (Sam) Wang, University of Missouri, St. Louis and Danforth Plant Science Center Increasing evidence indicates that environmental stresses, such as freezing, high salinity, and pathogen infection, lead to oxidative modification of plant membrane lipids to produce "ox-lipids". In contrast to oxylipins, such as jasmonic acid and its derivatives, whose significance in plant growth and defense against stress has been well documented, little is known about the functions of ox-lipids in plants. Ox-lipids may function as mediators signaling stress responses, they may represent damage that could serve as a protective buffer against oxidative damage elsewhere in the cell, or they may be long-term modifications that might function as stress "memory". Thus, ox-lipids have the potential to be essential mediators of plant response to the environment. The goals of the research project are to understand the role of ox-lipids in plant responses to biotic and abiotic stresses and to determine the function of members of two enzyme families, lipoxygenases and acyl hydrolases, which are likely to play important roles in the metabolism of oxidized lipids. The project will test the hypotheses that patterns of ox-lipids are fingerprints of individual stresses and that production and/or removal of specific ox-lipids by lipoxygenases and acyl hydrolases contributes to plant adaptation to stress. Under freezing and high salinity stress (abiotic stress) and infection by a fungal pathogen, Botrytis cinerea, and a bacterial pathogen, Pseudomonas syringae (biotic stresses), the stress-response phenotype and production of ox-lipids by wild-type plants and lipoxygenase- and acyl hydrolase-deficient mutant plants will be documented. The data will shed light on the roles of lipoxygenases and acyl hydrolases in stress responses and in production of specific ox-lipid patterns. Analysis of the stress-phenotype and ox-lipid profiles will lead to identification of ox-lipids that are candidates for mediating plant stress responses. The function of candidate lipid mediators will be tested by lipid analysis and phenotypic analysis of plants overexpressing enzymes that produce the candidate lipids and by supplementing mutant and wild-type plants with the putative mediators. The results have the potential to fill critical gaps in understanding of how lipid metabolic enzymes, cellular lipids, and their metabolites interact to influence plant performance. Broader Impacts: Carrying out the proposed work will provide training for multiple students and postdoctoral trainees at four institutions and bring current knowledge of metabolic profiling, functional genomics, and stress biology to the classroom. It will broaden the participation of underrepresented groups in research through the McNair Program at the University of North Texas, the Summer Undergraduate Research Opportunity Program at Kansas State University, the Des Lee Collaborative Scholarships at the University of Missouri, and the Danforth Plant Science Center NSF REU-Site program, which has achieved over 30% participation by underrepresented minority groups in the past several years. It will involve high school students in the research through the Texas Academy of Mathematics and Science at University of North Texas and through the Students and Teachers as Research Scientists (STARS) program in St. Louis. Organization of mass spectral data on plant lipids, and particularly on stress-induced lipids, into a web-accessible database will provide a foundation for further investigation of the structure and function of lipids, and particularly novel lipids, and will facilitate integration of lipidomics data with other metabolomics and functional genomics data. Analytical capabilities developed in this work will become enabling technologies available to researchers worldwide via the Kansas Lipidomics Research Center. This work also will provide insight into the identity of metabolic steps with potential to enhance stress tolerance in plants and improve agricultural productivity and quality.

植物胁迫反应中氧化膜脂的代谢谱和功能。Gadbury，堪萨斯州立大学Jyoti Shah，北德克萨斯大学Xuemin（Sam）Wang，密苏里州大学圣路易斯分校和丹佛斯植物科学中心越来越多的证据表明，环境胁迫，如冰冻、高盐和病原体感染，导致植物膜脂氧化修饰产生“ox-lipids”。与氧化脂质如茉莉酸及其衍生物相比，其在植物生长和防御胁迫中的重要性已被充分记载，对植物中氧化脂质的功能知之甚少。氧化脂质可能作为介质信号应激反应，他们可能代表损害，可以作为一个保护性缓冲区，防止氧化损伤在细胞中的其他地方，或者他们可能是长期的修改，可能作为应激“记忆”。因此，氧化脂质有可能成为植物对环境响应的重要介质。该研究项目的目标是了解氧化脂质在植物对生物和非生物胁迫的反应中的作用，并确定两种酶家族，脂氧合酶和酰基水解酶的成员的功能，这两种酶家族可能在氧化脂质的代谢中发挥重要作用。该项目将测试的假设，氧化脂质的模式是个人的压力和生产和/或特定的氧化脂质脂氧合酶和酰基水解酶的去除有助于植物适应压力的指纹。在冷冻和高盐胁迫（非生物胁迫）和真菌病原体灰葡萄孢和细菌病原体假单胞菌（生物胁迫）的感染下，将记录野生型植物和脂氧合酶和酰基水解酶缺陷突变体植物的胁迫响应表型和氧化脂质的产生。这些数据将阐明脂氧合酶和酰基水解酶在应激反应和特定氧化脂质模式产生中的作用。胁迫表型和氧化脂质谱的分析将导致氧化脂质介导植物胁迫反应的候选人的鉴定。候选脂质介质的功能将通过脂质分析和过表达产生候选脂质的酶的植物的表型分析以及通过用推定的介质补充突变体和野生型植物来测试。这些结果有可能填补关键空白，了解脂质代谢酶，细胞脂质及其代谢产物如何相互作用，影响植物的性能。更广泛的影响：开展拟议的工作将为四个机构的多名学生和博士后学员提供培训，并将代谢分析，功能基因组学和压力生物学的现有知识带入课堂。它将通过北德克萨斯大学的McNair计划、堪萨斯州立大学的夏季本科生研究机会计划、密苏里州大学的Des Lee合作奖学金以及丹福思植物科学中心NSF REU-Site计划来扩大代表性不足的群体对研究的参与，该计划在过去几年中使代表性不足的少数群体的参与率超过30%。它将通过北德克萨斯大学的德克萨斯数学和科学学院以及圣路易斯的学生和教师作为研究科学家（STARS）计划让高中生参与研究。组织质谱数据的植物脂质，特别是胁迫诱导的脂质，到一个网络访问的数据库将提供一个基础，进一步调查的结构和功能的脂质，特别是新的脂质，并将促进整合的lipidomics数据与其他代谢组学和功能基因组学数据。在这项工作中开发的分析能力将通过堪萨斯脂质组学研究中心成为全球研究人员可用的技术。这项工作还将提供对代谢步骤的识别的深入了解，这些代谢步骤有可能增强植物的胁迫耐受性，提高农业生产力和质量。