RUI: Analysis of the Role of a Family of Putative E3 Ubiquitin-Ligase Target Adapters in Phytochrome Signaling in Arabidopsis thaliana

RUI：推定 E3 泛素连接酶靶标适配器家族在拟南芥光敏色素信号转导中的作用分析

• 批准号: 0919678
• 负责人: Derek Gingerich
• 金额: $ 15万
• 依托单位: University of Wisconsin-Eau Claire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919678&HistoricalAwards=false
• 关键词: RUI; Analysis; Role; Family; Putative

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).The ability to perceive and respond to light is vital to plant growth and development. Previous work has identified two genes (LRB1 and LRB2) in the model plant Arabidopsis thaliana that have roles in light responses. Plants with disruptions of these two genes are more shade tolerant, a result of increased sensitivity to red wavelength light. Analysis of the sequences of the LRB genes suggest that they act by regulating the breakdown of other proteins, therefore the likely role of the LRBs in plant cells is to modulate the levels of a protein or proteins that participate in red light perception/response. This project will use a variety of standard molecular, genetic, and biochemical approaches to more fully elucidate the role of LRB genes in plant shade responses by determining where in red light-response pathway the LRB genes act, identifying the proteins that are regulated by LRB1 and LRB2, and investigating the structure of the molecular complexes of which the proteins which are encoded by the LRB genes are a part. Shade responses are important in agriculture, as shading can reduce agronomic performance by increasing lodging (falling over) or decreasing yield, therefore the information obtained in this project may help guide future work on light responses in crop plants. Also, the majority of work in this project will be conducted by undergraduate students at the University of Wisconsin-Eau Claire (a four-year public comprehensive university), significantly enhancing their education and making them more competitive and better prepared as they enter graduate/professional school or the workforce.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。感知和响应光线的能力对植物生长和发育至关重要。以前的工作已经确定了两个基因（LRB 1和LRB 2）在模式植物拟南芥中的光响应的作用。这两个基因被破坏的植物更耐荫，这是对红色波长光敏感性增加的结果。对LRB基因序列的分析表明，它们通过调节其他蛋白质的分解来起作用，因此LRB在植物细胞中的可能作用是调节参与红光感知/响应的一种或多种蛋白质的水平。该项目将使用各种标准的分子、遗传和生物化学方法，通过确定LRB基因在红光响应途径中的作用，鉴定由LRB 1和LRB 2调节的蛋白质，并研究LRB基因编码的蛋白质是其一部分的分子复合物的结构，来更全面地阐明LRB基因在植物遮荫响应中的作用。 遮荫反应在农业中很重要，因为遮荫可以通过增加倒伏（跌倒）或降低产量来降低农艺性能，因此本项目中获得的信息可能有助于指导未来作物对光反应的研究。此外，该项目的大部分工作将由威斯康星大学欧克莱尔分校（一所四年制公立综合大学）的本科生进行，大大提高他们的教育水平，使他们在进入研究生/专业学校或劳动力市场时更具竞争力和更好的准备。