RUI: Analysis of Mutants Identified in Screens for Suppressors or Enhancers of a Red Light Hypersensitive E3 Ubiquitin-Ligase Mutant

RUI：对红光超敏 E3 泛素连接酶突变体的抑制子或增强子筛选中鉴定的突变体进行分析

• 批准号: 1354438
• 负责人: Derek Gingerich
• 金额: $ 21.45万
• 依托单位: University of Wisconsin-Eau Claire
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1354438&HistoricalAwards=false
• 关键词: RUI; Analysis; Mutants; Identified; Screens

The ability to perceive and respond to light is vital to plant growth and development. Previous work by the Principal Investigator (PI) has identified and characterized two genes (LRB1 and LRB2) in the model flowering plant Arabidopsis thaliana that regulate responses to red wavelength light. Mutant plants with disruptions of these two genes are, among other things, more shade tolerant. The PI has used these mutants as a basis for novel genetic screens intended to identify additional genes that have roles in light perception and response. This project will use a variety of standard molecular, genetic, and biochemical approaches to study these genes. By identifying and characterizing genes which had not been previously implicated in light responses, this project will provide new insights into the mechanisms by which plants respond to their light environment. Shade responses are important in agriculture, as shading can reduce agronomic performance by increasing lodging (falling over), decreasing yield, and reducing plant defenses to pathogens and pests, therefore the information obtained in this project may help guide future work on light responses in crop plants. Also, the majority of work in this project will be conducted by undergraduate students at the University of Wisconsin-Eau Claire (a four-year public comprehensive university), significantly enhancing their education and making them more competitive and better prepared as they enter graduate/professional school or the workforce.

感知和响应光的能力对于植物的生长和发育至关重要。首席研究员 (PI) 之前的工作已在模型开花植物拟南芥中鉴定并表征了两个基因（LRB1 和 LRB2），它们调节对红色波长光的反应。除其他外，破坏这两个基因的突变植物具有更强的耐荫性。 PI 使用这些突变体作为新型基因筛选的基础，旨在识别在光感知和反应中发挥作用的其他基因。该项目将使用各种标准分子、遗传和生化方法来研究这些基因。通过识别和表征以前与光反应无关的基因，该项目将为植物对其光环境做出反应的机制提供新的见解。遮荫反应在农业中很重要，因为遮荫会通过增加倒伏（倒伏）、降低产量和减少植物对病原体和害虫的防御来降低农艺性能，因此本项目中获得的信息可能有助于指导未来作物植物光反应的工作。此外，该项目的大部分工作将由威斯康星大学欧克莱尔分校（一所四年制公立综合性大学）的本科生完成，这将显着提高他们的教育水平，使他们在进入研究生/职业学校或劳动力市场时更具竞争力并做好更好的准备。