The roles of the AMP-activated kinase in metabolic homeostasis in Drosophila

AMP 激活激酶在果蝇代谢稳态中的作用

• 批准号: 0920443
• 负责人: Erik Johnson
• 金额: $ 33.03万
• 依托单位: Wake Forest University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920443&HistoricalAwards=false
• 关键词: roles; AMP; activated; kinase; metabolic

Food availability is a dynamic aspect of most environments and places significant challenges on organisms to maintain constant energy stores. The consequences of energy shortage include changes in behavior and physiology that reflect changes in the allocation of energy. Part of the decision process on how energy is utilized depends upon the actions of the AMP-activated kinase, a highly-conserved molecule that becomes active during energy shortages. It is also clear that different cellular populations have differential responses during energy deprivation, although it is unclear the extent that the AMP-activated kinase partakes in these processes. This project takes advantage of the unique molecular and genetic tools available in the fruit fly, Drosophila melanogaster, to investigate how the AMP-activated kinase regulates behavioral and physiological changes during starvation conditions. This research will contribute new information regarding the cellular signals within defined neural and peripheral tissues that are responsible for generating specific behavioral and physiological changes to maintain energy homeostasis. The evolutionary conservation of this particular molecule, as well as the conservation of behavioral responses to starvation, indicates results of this research will have broad applications, from pest management to the development of potential novel therapeutics. In addition to offering insight into the cell-specific roles of the AMP-activated kinase, this research project will be used as a vehicle to extend research opportunities to undergraduate students and enhance professional development of faculty at a primarily undergraduate institution, Francis Marion University. Lastly, this project will involve the training of graduate students.

粮食可用性是大多数环境的动态方面，并且在生物体上构成了恒定能量存储的重大挑战。能量短缺的后果包括反映能量分配变化的行为和生理学的变化。关于如何利用能量的决策过程的一部分取决于AMP激活激酶的作用，AMP激活激酶是一种高度保存的分子，在能量短缺期间变得活跃。同样很明显，在能量剥夺期间，不同的细胞群体具有差异反应，尽管目前尚不清楚AMP激活的激酶参与这些过程的程度。该项目利用了果蝇果蝇中可用的独特分子和遗传工具，研究了AMP激活的激酶如何调节饥饿条件下的行为和生理变化。这项研究将为定义的神经和周围组织中的细胞信号提供新的信息，这些信息负责产生特定的行为和生理变化以维持能量稳态。该特定分子的进化保护以及对饥饿的行为反应的保护表明，这项研究的结果将具有广泛的应用，从害虫管理到潜在的新型治疗剂的发展。除了洞悉AMP激活激酶的细胞特异性角色外，该研究项目还将用作扩展研究机会的工具，并在弗朗西斯·马里恩大学（Francis Marion University）的一家本科生中扩大教师的专业发展。最后，该项目将涉及研究生的培训。