Comprehensive Analysis of Physiological Capabilities, Ecological Functions and Ecological Niches of Acidophilic Microorganisms

嗜酸微生物生理能力、生态功能和生态位的综合分析

• 批准号: 193860825
• 负责人: Professor Dr. Johannes Gescher
• 金额: --
• 依托单位: Institut für Technische Mikrobiologie V-7
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/193860825?language=en
• 关键词: Comprehensive; Analysis; Physiological; Capabilities; Ecological

Here we propose to investigate a stalactite-like bacterial community from an abandoned pyrite mine in Elbingerode (Germany). The community is fueled exclusively by the decomposition of pyrite (FeS2) and is embedded in a mixture of extracellular polysaccharides and products of secondary mineraliza-tion events. The majority of bacterial species that live in and build up this spatially confined environ-ment are dependent on the catalysis of iron oxidation and reduction reactions. Our proposal aims at dissecting the biogeochemical and environmental factors that contribute to the formation of ecological niches for different iron oxidizing and reducing bacteria as well as a novel group of archaea with so far undiscovered physiological capabilities. We strive to understand these factors in this particular biofilm due to its spatially highly limited location and the moderate number of microbial species present. These attributes give the community a model character for a multitude of acid rock drainage dependent consortia. Integration of results from bio/geochemical characterization and metagenomic and metaproteomic analysis with fluorescent in situ hybridization (FISH) experiments using probes towards different iron oxidizing and reducing organisms and archaea will lead to a model for the ecological niches and the potential interspecies dependence within the biofilm. These models will be tested with enrichment and isolation experiments in which we will mimic the environmental niches of different bacterial and archaeal strains to isolate them from the bacterial community.

在这里，我们建议调查一个钟乳石样细菌群落从一个废弃的黄铁矿在elbingerod（德国）。该群落完全由黄铁矿（FeS2）分解提供燃料，并嵌入细胞外多糖和二次矿化事件产物的混合物中。大多数细菌种类生活和建立这种空间受限的环境依赖于铁的氧化和还原反应的催化。我们的建议旨在剖析生物地球化学和环境因素，这些因素有助于形成不同的铁氧化和还原细菌的生态位，以及一种迄今为止尚未发现的具有生理能力的新古菌群。我们努力了解这些因素在这种特殊的生物膜，由于其空间高度有限的位置和适度数量的微生物物种存在。这些特征使该群落成为众多依赖酸性岩石排水的群落的典型特征。将生物/地球化学表征、宏基因组学和元蛋白质组学分析结果与荧光原位杂交（FISH）实验结合起来，利用探针对不同的铁氧化和还原生物和古细菌进行研究，将建立一个生态位模型和生物膜内潜在的种间依赖关系。这些模型将通过富集和分离实验进行测试，我们将模拟不同细菌和古细菌菌株的环境生态位，从细菌群落中分离出它们。