Establishing jet loop reactors as scalable bioelectrochemical reactor systems for anodic and cathodic production processes

建立喷射环流反应器作为阳极和阴极生产过程的可扩展生物电化学反应器系统

• 批准号: 445800740
• 负责人: Professor Dr. Johannes Gescher
• 金额: --
• 依托单位: Institut für Mehrphasenströmungen V-5
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/445800740?language=en
• 关键词: Establishing; jet; loop; reactors; scalable

Bioelectrochemical reactor systems are typically surface limited. It is the amount of electrode surface area per reactor volume that is key for advancing space-time-yields. At the same time the electrode surface and the electroactive organisms building a biofilm on the electrodes have to be seen as one composite material that can be advanced regarding the interaction of its components. Last but not least it is challenging to construct these surface dependent reactor systems in a way that dead volumes are avoided and the architecture of the living whole cell biocatalyst is steered towards process optimization. To address these topics we will engineer a scalable bioelectrochemical jet loop reactor concept and will show its versatility by operating it as microbial electrolysis as well as bioelectrosynthesis platform. In the bioelectrosynthesis research direction we will operate it with the recently isolated extremophile Kyrpidia spormannii and aim to produce biomass and the biopolymer polyhydroxybutyrate from carbon dioxide and electrical energy. In the microbial electrolysis research direction, we will increase reactor performance by producing synthetic conductive nanowires as well as conductive direct cell-cell connections. To this end we will use Shewanella oneidensis as well as a currently developed Escherichia coli strain as model organism and produce the platform chemical acetoin in an anode assisted fermentation. In both reactor configurations we will be able to benefit from additive manufacturing of 3D-electrodes as well as their functionalization by conductive polymers.

生物电化学反应器系统通常是表面受限的。单位反应器体积的电极表面积是提高时空产率的关键。同时，电极表面和在电极上构建生物膜的电活性生物体必须被视为一种复合材料，该复合材料可以关于其组分的相互作用而被推进。最后但并非最不重要的是，以避免死体积并且将活的全细胞生物催化剂的架构转向过程优化的方式构建这些表面依赖性反应器系统是具有挑战性的。为了解决这些问题，我们将设计一个可扩展的生物电化学喷射回路反应器概念，并将通过将其作为微生物电解和生物电合成平台来展示其多功能性。在生物电合成研究方向，我们将使用最近分离的极端微生物Kyrpidia spormannii进行操作，旨在从二氧化碳和电能中生产生物质和生物聚合物聚羟基丁酸酯。在微生物电解研究方向，我们将通过生产合成导电纳米线以及导电直接细胞-细胞连接来提高反应器性能。为此，我们将使用希瓦氏菌oneidensis以及目前开发的大肠杆菌菌株作为模式生物，并在阳极辅助发酵中产生平台化学品乙偶姻。在这两种反应器配置中，我们将能够受益于3D电极的增材制造以及导电聚合物的功能化。