Physical Regulation of Microtubule Biomechanics

微管生物力学的物理调节

• 批准号: 0928540
• 负责人: Jennifer Ross
• 金额: $ 35万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0928540&HistoricalAwards=false
• 关键词: Physical; Regulation; Microtubule; Biomechanics

The goal of this research is to understand and control the mechanical properties of the cytoskeletal filament called microtubules. Microtubules are nano-scale filaments, and the mechanical properties have ramifications for the shape of cells, cell division, and cell motility. Understanding how the mechanics of single microtubules is controlled is essential for creating an accurate quantitative and predictive model to relate the strength of single microtubules to the strength of the entire cytoskeletal network. This work will systematically measure and model how lattice defects, associated proteins, and post-translational modifications can affect microtubule rigidity. This work represents the first systematic study of how the microtubule lattice can directly affect the mechanics of single microtubules.The proposed approach will reveal new information about how the structure of microtubules can affect its mechanics. The results will have an impact on basic cell biology, since microtubules are essential for many cellular processes. Moreover, microtubules are an entropically-driven, self-assembled system made from identical protein subunits. Elucidating how mechanical properties of this system can be altered by lattice defects, external binding partners, and modifications to the subunits will have ramifications for other self-assembled condensed matter systems made of colloids, polymers, and proteins. Microtubules and their related proteins can be harnessed as a biological-scaffold for nano-scale assemblies. This work will enable bio-engineering of novel bio-memetic materials from the microtubule-cytoskeletal system. Determining the mechanical properties of the individual microtubule structure is essential for future engineering processes that may involve them. The research proposed here is truly interdisiciplinary, combining the fields of condensed matter physics, bio-engineering, materials science, and cell biology.

这项研究的目的是了解和控制被称为微管的细胞骨架细丝的机械性能。微管是纳米级的细丝，其力学性能对细胞的形状、细胞分裂和细胞运动都有影响。了解单个微管的力学是如何控制的，对于建立一个准确的定量和预测模型将单个微管的强度与整个细胞骨架网络的强度联系起来是至关重要的。这项工作将系统地测量和模拟晶格缺陷、相关蛋白质和翻译后修饰如何影响微管刚性。这项工作首次系统地研究了微管晶格如何直接影响单个微管的力学性能。所提出的方法将揭示微管结构如何影响其力学性能的新信息。这一结果将对基础细胞生物学产生影响，因为微管对许多细胞过程都是必不可少的。此外，微管是一个由相同的蛋白质亚基组成的熵驱动的自组装系统。阐明这一体系的力学性质如何因晶格缺陷、外部结合伙伴和亚基的修饰而改变，这将对其他由胶体、聚合物和蛋白质组成的自组装凝聚体系统产生影响。微管及其相关蛋白质可以作为纳米级组装的生物支架。这项工作将使来自微管-细胞骨架系统的新型生物模因材料的生物工程成为可能。确定单个微管结构的机械性能对于未来可能涉及到它们的工程过程是至关重要的。这里提出的研究是真正的跨学科，结合了凝聚态物理、生物工程、材料科学和细胞生物学等领域。