EAGER: Nanoparticle-Based Integrated Microsystem for Selective Targeting of Bio-Species in Flowing Suspensions

EAGER：基于纳米颗粒的集成微系统，用于选择性靶向流动悬浮液中的生物物种

• 批准号: 0943321
• 负责人: Yitshak Zohar
• 金额: $ 14.99万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0943321&HistoricalAwards=false
• 关键词: EAGER; Nanoparticle; Based; Integrated; Microsystem

The objective of this research is to develop a high-performance system to target individual bio-species with dramatically enhanced specificity and sensitivity. The approach is based on utilizing smart nanoparticles, encapsulating various chemicals and equipped with homing features, to provide a vehicle for pin-point delivery of payloads at a nanoscale with high efficiency. Current systems utilize drugs with very low specificity resulting in serious side effects and poor system response. Organic-inorganic nanoparticles will be functionalized with antibodies for specific binding to circulating tumor cells flowing in complex mixtures to improve the system performance.The intellectual merit lies in the goal of selectively capturing a rare moving bio target that is still a tremendous engineering challenge. Metastatic cancer cells circulating in the blood stream will be targeted. To meet this challenge, unique trans-membrane cell-cell adhesion molecules are used as receptors. The antibodies on the hybrid nanoparticles, having a liposomal bilayer structure and a silicate surface, are used as homing ligands for selective binding to the target cell receptors.The project could have a major impact by applying the proposed methodologies not only to detection but also diagnosis, monitoring and treatment of diseases. An automated immunoassay employed at the point-of-care with enhanced specificity will increase the efficacy of patient treatment helping eradicate diseases such as cancer. The proposed project directly supports the Bio-MEMS educational program currently under development; it incorporates workforce development by providing an opportunity for students to carry out advanced interdisciplinary research. Science education outreach for K-12 and general communities will also be arranged.

这项研究的目标是开发一种高性能的系统，以显著增强的特异性和敏感性来针对单个生物物种。该方法基于利用智能纳米颗粒，封装各种化学品并配备寻的功能，以提供一种高效率地在纳米级点状输送有效载荷的工具。目前的系统使用的药物特异性很低，导致严重的副作用和较差的系统响应。有机-无机纳米粒子将与抗体功能化，以与流动在复杂混合物中的循环肿瘤细胞特异性结合，以提高系统性能。智能优势在于选择性地捕获罕见的移动生物目标，这仍然是一个巨大的工程挑战。循环在血流中的转移性癌细胞将成为目标。为了迎接这一挑战，独特的跨膜细胞-细胞黏附分子被用作受体。具有脂质体双层结构和硅酸盐表面的杂化纳米颗粒上的抗体被用作寻的配体，选择性地结合到靶细胞受体上。该项目不仅可以应用于检测，而且可以应用于疾病的诊断、监测和治疗。在护理地点采用的自动化免疫分析具有更高的特异性，将提高患者治疗的效率，有助于根除癌症等疾病。拟议的项目直接支持目前正在开发的生物微机械教育计划；它通过为学生提供开展高级跨学科研究的机会，纳入了劳动力发展。还将为K-12和一般社区安排科学教育外展。