The Complete Genome Sequence of the Glaucophyte Alga Cyanophora paradoxa

灰藻藻 Cyanophora paradoxa 的完整基因组序列

• 批准号: 0946528
• 负责人: Debashish Bhattacharya
• 金额: $ 26.3万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0946528&HistoricalAwards=false
• 关键词: Complete; Genome; Sequence; Glaucophyte; Alga

How was photosynthesis established in eukaryotes? To gain insights into this fundamental step in the evolution of our planet, a grant has been awarded to Dr. Debashish Bhattacharya at the University of Iowa and Dr. Jeffrey Boore at SymBio Corporation to determine to high coverage the 140 million base pair nuclear genome sequence of the unicellular alga Cyanophora paradoxa. Cyanophora is a member of the remaining group of photosynthetic eukaryotes (Glaucophyta) that still lacks a complete genome sequence. The single cyanobacterial primary endosymbiosis that gave rise to all plastids (e.g., chloroplasts) occurred in the common ancestor of Cyanophora, other algae, and plants. This was a pivotal and ancient (~1.5 billion years ago) event in the Earth's history that laid the foundation for modern terrestrial ecosystems. A critical step in plastid establishment was the transfer of endosymbiont genes to the "host" nucleus. It is unclear however whether this massive transfer was limited to genes strictly involved in plastid metabolism or whether the host profited from the captured genome to explore other novel functions via recruitment of genes from the cyanobacterium. The Cyanophora genome sequence will enable scientists to rigorously test this idea in a relatively "simple" algal model. Beyond its contribution to understanding endosymbiosis, the Cyanophora genome sequence will allow countless other insights which include identifying a set of core genes shared by algae and plants that can be studied in detail to understand the origin of plant-specific characters. In addition the Cyanophora genome will be invaluable for guiding the annotation of the genomes of plants and other protists.Although this is a basic science project, the Cyanophora project has the potential to accelerate education and interest at all levels in protists and their genomes. The research team will incorporate the findings of this study into several outreach efforts. The principles of high throughput genomics will be incorporated into several courses offered at the University of Iowa as well as in the NSF-funded undergraduate summer "Workshop on Comparative Evolutionary Genomics", the findings of the study will be published in the Microbial Life Educational Resources web page at the micro*scope web site http://starcentral.mbl.edu/microscope/portal.php, two graduate students and several undergraduates will be trained at the University of Iowa, and the PI will recruit one or more minority summer student per year through the AGEP program at Iowa.

光合作用是如何在真核生物中建立的？为了深入了解我们星球进化的这一基本步骤，爱荷华州大学的Deaderish Bhattacharya博士和SymBio公司的Jeffrey Boore博士获得了一笔赠款，以确定单细胞蓝藻的1.4亿碱基对核基因组序列的高覆盖率。蓝藻属是光合真核生物（蓝藻门）的一个成员，仍然缺乏完整的基因组序列。单一的蓝藻初级内共生产生了所有的质体（例如，叶绿体）发生在蓝藻属、其他藻类和植物的共同祖先中。这是地球历史上一个重要而古老的事件（约15亿年前），为现代陆地生态系统奠定了基础。质体建立的一个关键步骤是将内共生体基因转移到“宿主”细胞核。然而，目前尚不清楚这种大规模转移是否仅限于严格参与质体代谢的基因，或者宿主是否从捕获的基因组中获益，以通过从蓝细菌中招募基因来探索其他新功能。蓝细菌基因组序列将使科学家能够在相对“简单”的藻类模型中严格测试这一想法。除了对理解内共生的贡献外，蓝藻基因组序列还将提供无数其他见解，包括确定藻类和植物共有的一组核心基因，可以详细研究这些基因，以了解植物特异性特征的起源。此外，蓝藻基因组将是非常宝贵的指导注释的基因组的植物和其他protists.虽然这是一个基础科学项目，蓝藻项目有可能加快教育和兴趣，在各个层次的原生生物和他们的基因组.研究小组将把这项研究的结果纳入若干外联工作。高通量基因组学的原理将被纳入爱荷华州大学提供的几门课程中，以及NSF资助的本科生暑期“比较进化基因组学研讨会”中，研究结果将发表在micro*scope网站http：//starcentral.mbl.edu/microscope/portal.php，两名研究生和几名本科生将在爱荷华州大学接受培训，PI将通过爱荷华州的AGEP项目每年招收一名或多名少数民族暑期学生。